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首页> 外文期刊>Cellular Oncology: Analytical Cellular Pathology >Correlation of Grade of Urothelial Cell Carcinomas and DNA Histogram Features Assessed by Flow Cytometry and Automated Image Cytometry
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Correlation of Grade of Urothelial Cell Carcinomas and DNA Histogram Features Assessed by Flow Cytometry and Automated Image Cytometry

机译：流式细胞仪和自动图像细胞仪评估的尿道上皮细胞癌分级与DNA直方图特征的相关性

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Objective: To analyse how DNA ploidy and S-phase fraction (SPF) by flow cytometry (FCM) and an optimised fullyautomatic DNA image cytometer (ICM) correlate with grade in TaT1 urothelial cell carcinomas (UC) of the urinary bladder.Materials and methods: Two-hundred-and twenty-eight consensus cases were analysed. Single cell suspensions were stained(DAPI for FCM, Feulgen for ICM). There was enough material for both FCMand ICMin 202 of these cases. FCMand optimisedICM measurements were performed on the 202 UCs. To discriminate between different grades, single- and multivariate analyseswas performed on DNA histogram features obtained with the MultiCycle program (using DNA index (DI) and SPF). Results:Overall measurement time of the adapted ICM method was 10.7 minutes per case (range 5.9–29.8 min.) and required littleadditional interactive object rejection (average 152 objects (84–298) on 3000 objects per case measured, which took 9.9 minuteson average, range 8.3–15.5 minutes). The ICM histograms looked much “cleaner” with less noise than the FCM graphs. Thecoefficient of variation (CV) of the diploid peak for ICM(5.4%) was significantly lower than for FCM(5.9%) (p< 0.0001). ICMfeatures were more strongly correlated to grade than FCMfeatures. In multivariate analysis, the best discriminating set of featureswas DNA ploidy and SPF (both by ICM).Conclusions: The adapted fully automated DNA ICM works very well for UCs. LowCV DNA ICM histograms are obtained in a time comparable to FCM. The DNA ICM results have stronger discriminative powerthan DNA FCM for grade in TaT1 UCs. Colour figures can be viewed onhttp://www.esacp.org/acp/2003/25-3/bol.htm.
机译：目的：通过流式细胞仪（FCM）和优化的全自动DNA图像细胞仪（ICM）分析DNA倍性和S期分数（SPF）与膀胱TaT1尿路上皮细胞癌（UC）的等级之间的关系。 ：分析了288例共识案例。对单细胞悬液进行染色（DAPI用于FCM，Feulgen用于ICM）。对于这些案例，FCM和ICMin 202都有足够的材料。在202个UC上进行了FCM和优化的ICM测量。为了区分不同的等级，对通过MultiCycle程序（使用DNA索引（DI）和SPF）获得的DNA直方图特征进行了单变量和多变量分析。结果：采用ICM方法的总测量时间为每例10.7分钟（范围为5.9–29.8分钟），几乎不需要其他交互式物体剔除（每箱平均3000个物体上平均152个物体（84–298）），平均耗时9.9分钟。 ，范围8.3–15.5分钟）。与FCM图相比，ICM直方图看起来“更干净”且噪声更少。 ICM（5.4％）的二倍体峰变异系数（CV）明显低于FCM（5.9％）（p <0.0001）。与FCM功能相比，ICM功能与等级的相关性更高。在多变量分析中，最能区分特征的是DNA倍性和SPF（均由ICM得出）。结论：经过改编的全自动DNA ICM对UC非常有效。 LowCV DNA ICM直方图在与FCM相当的时间内获得。在TaT1 UC中，DNA ICM结果具有比DNA FCM更高的判别力。可以在http://www.esacp.org/acp/2003/25-3/bol.htm上查看彩色图形。

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