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首页> 外文期刊>Cell discovery. >Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation
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Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation

机译：大鼠支持细胞成熟过程中DNA 5-羟甲基胞嘧啶的全基因组分析

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Sertoli cells have dual roles during the cells’ lifetime. In the juvenile mammal, Sertoli cells proliferate and create the structure of the testis, and during puberty they cease to proliferate and take on the adult role of supporting germ cells through spermatogenesis. Accordingly, many genes expressed in Sertoli cells during testis formation are repressed during spermatogenesis. 5-Hydroxymethylcytosine (5hmC) is a DNA modification enzymatically generated from 5mC and present in all investigated mammalian tissues at varying levels. Using mass spectrometry and immunofluorescence staining we identified a substantial Sertoli cell-specific global 5hmC increase during rat puberty. Chemical labeling, pull-down and sequencing of 5hmC-containing genomic DNA from juvenile and adult rat Sertoli cells revealed that genes that lose or gain 5hmC belong to different functional pathways and mirror the functions of the cells in the two different states. Loss of 5hmC is associated with genes involved in development and cell structure, whereas gain of 5hmC is associated with genes involved in cellular pathways pertaining to the function of the adult Sertoli cells. This redistribution during maturation shows that 5hmC is a dynamic nucleotide modification, correlated to gene expression.
机译：Sertoli细胞在细胞的生命周期中具有双重作用。在幼年哺乳动物中，Sertoli细胞增殖并形成睾丸结构，在青春期，它们停止增殖，并通过成精作用担负起支持生殖细胞的成年作用。因此，在睾丸形成过程中在支持细胞中表达的许多基因在精子发生过程中受到抑制。 5-羟甲基胞嘧啶（5hmC）是从5mC酶促产生的DNA修饰，并以不同的水平存在于所有研究的哺乳动物组织中。使用质谱和免疫荧光染色，我们确定了大鼠青春期期间大量的Sertoli细胞特异性全局5hmC增加。来自少年和成年大鼠支持细胞的含5hmC基因组DNA的化学标记，下拉和测序表明，丢失或获得5hmC的基因属于不同的功能途径，并且反映了两种不同状态下细胞的功能。 5hmC的丧失与参与发育和细胞结构的基因有关，而5hmC的增加与参与与成年支持细胞功能有关的细胞途径的基因有关。成熟过程中的这种重新分布表明5hmC是一种动态核苷酸修饰，与基因表达相关。

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