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首页> 外文期刊>Catalysts >Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review
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Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

机译：非水反应系统中的酶稳定性和活性：迷你回顾。

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Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P , functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation.
机译：由于良好的对映选择性，逆热力学平衡和无水依赖性副反应的优点，最近引起了对非水相生物催化的极大兴趣。已经证明酶在非水介质中具有高活性和稳定性，并且酶活性的变化归因于其构象修饰。这篇综述全面论述了完整酶在各种非水体系中的稳定性和活性，例如有机溶剂，离子液体，亚/超临界流体及其组合混合物。已经发现关键因素，例如Log P，官能团和溶剂的分子结构定义了围绕酶分子的微环境并影响酶的三级和二级结构，影响酶的催化性能。因此，阐明在酶周围的微环境与其稳定性和活性之间的联系对于在非水介质中进行生物催化非常重要。实际上，更好地理解不同的非水环境与酶结构，稳定性和活性之间的相关性可以有助于确定对于给定的生物转化最合适的反应介质。

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