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首页> 外文期刊>Cell Division >Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles
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Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles

机译：果蝇S2细胞的后期B纺锤体动力学：与胚胎纺锤体的比较

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Background In the Drosophila melanogaster syncytial blastoderm stage embryo anaphase B is initiated by a cell cycle switch in which the suppression of microtubule minus end depolymerization and spatial reorganization of the plus ends of outwardly sliding interpolar microtubules triggers spindle elongation. RNA interference in Drosophila cultured S2 cells may present a useful tool for identifying novel components of this switch, but given the diversity of spindle design, it is important to first determine the extent of conservation of the mechanism of anaphase B in the two systems. Results The basic mechanism, involving an inverse correlation between poleward flux and spindle elongation is qualitatively similar in these systems, but quantitative differences exist. In S2 cells, poleward flux is only partially suppressed and the rate of anaphase B spindle elongation increases with the extent of suppression. Also, EB1-labelled microtubule plus ends redistribute away from the poles and towards the interpolar microtubule overlap zone, but this is less pronounced in S2 cells than in embryos. Finally, as in embryos, tubulin FRAP experiments revealed a reduction in the percentage recovery after photobleaching at regions proximal to the pole. Conclusions The basic features of the anaphase B switch, involving the suppression of poleward flux and reorganization of growing microtubule plus ends, is conserved in these systems. Thus S2 cells may be useful for rapidly identifying novel components of this switch. The quantitative differences likely reflect the adaptation of embryonic spindles for rapid, streamlined mitoses.
机译：背景技术在果蝇的合胞体胚盘期胚胎后期B是由细胞周期开关启动的，其中抑制微管减去末端解聚和向外滑动的极间微管正端的空间重组触发纺锤体伸长。果蝇培养的S2细胞中的RNA干扰可能是鉴定该开关新成分的有用工具，但是鉴于纺锤体设计的多样性，重要的是首先确定两个系统中后期B机理的保守程度。结果在这些系统中，涉及极通量与主轴伸长率成反比的基本机理在质量上相似，但存在数量差异。在S2细胞中，极向通量仅被部分抑制，后期B纺锤体伸长率随抑制程度的增加而增加。同样，EB1标记的微管正末端从极点向极间微管重叠区重新分布，但这在S2细胞中没有在胚胎中那么明显。最后，与胚胎一样，微管蛋白FRAP实验表明，在极点附近的区域进行光漂白后，回收率降低了。结论在这些系统中保留了后期B开关的基本特征，包括抑制极通量和重组生长的微管及末端。因此，S2细胞可用于快速识别该开关的新颖成分。数量差异可能反映了胚胎纺锤体对快速，简化的有丝分裂的适应。

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