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Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy

机译：使用光片显微镜在3D大球形肿瘤模型中进行活细胞分裂动力学监测

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Background Multicellular tumor spheroids are models of increasing interest for cancer and cell biology studies. They allow considering cellular interactions in exploring cell cycle and cell division mechanisms. However, 3D imaging of cell division in living spheroids is technically challenging and has never been reported. Results Here, we report a major breakthrough based on the engineering of multicellular tumor spheroids expressing an histone H2B fluorescent nuclear reporter protein, and specifically designed sample holders to monitor live cell division dynamics in 3D large spheroids using an home-made selective-plane illumination microscope. Conclusions As illustrated using the antimitotic drug, paclitaxel, this technological advance paves the way for studies of the dynamics of cell divion processes in 3D and more generally for the investigation of tumor cell population biology in integrated system as the spheroid model.
机译：背景技术多细胞肿瘤球体是对癌症和细胞生物学研究越来越感兴趣的模型。他们允许在探索细胞周期和细胞分裂机制时考虑细胞相互作用。但是，活球体中细胞分裂的3D成像在技术上具有挑战性，从未有过报道。结果在此，我们报告了基于表达组蛋白H2B荧光核报道蛋白的多细胞肿瘤球体工程学的重大突破，并特别设计了样品夹，以使用自制的选择性平面照明显微镜监测3D大球体中的活细胞分裂动力学。结论如使用抗有丝分裂药物紫杉醇所说明的那样，这项技术进步为研究3D细胞分裂过程的动力学铺平了道路，更广泛的意义是为集成系统（作为球体模型）研究肿瘤细胞群体生物学铺平了道路。

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