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首页> 外文期刊>Cellular Physiology and Biochemistry >Short-Term Hyperosmolality Pretreatment on Cells Can Reduce the Radiosensitivity via RVI and Akt1 Activation
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Short-Term Hyperosmolality Pretreatment on Cells Can Reduce the Radiosensitivity via RVI and Akt1 Activation

机译：短期高渗预处理可通过RVI和Akt1激活降低放射敏感性

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Background/Aims: The ionizing radiation (IR) has been applied in clinical treatment for many years and the radiosensitivity is crucial to the treatment. Radiosensitivity of cells is subjected to many environmental factors, such as hypoxia and temperature. Hyperosmolality as a common environmental factor has been demonstrated to be associated with survival and apoptosis of cells in many studies. Thus we investigated the influence of hyperosmolality on cells radiosensitivity. Methods: We examined the viability and surviving fraction of L-O2 cells of irradiated L-O2 cells, and detected the effect on AHH-1 cells by flow cytometry, in order to investigate the effect of short-term hyperosmolality pretreatment on cells radiosensitivity. Comet assay was used to assess the DNA strand breaks. Then the detection of Akt1 by western blot and the process of regulatory volume increase by CYSY-TT were involved in the mechanism. Result: We demonstrated that a short-term hyperosmolality pretreatment on cells could reduce their radiosensitivity. Further research indicated that the short-term hypertonic condition could induce regulatory volume increase (RVI), which activated Akt1 and degenerated the IκB-α. This process was associated with reduced cells radiosensitivity. Finally, we used the flufenamic acid (FFA), a blocker to cation channels (HICCs) to inhabit RVI and consequently inhabit the protective effect of hyperosmolality on irradiated cells. Conclusion: a short-term hyperosmolality pretreatment could reduce the cells radiosensitivity by RVI and following activation of Akt1.
机译：背景/目的：电离辐射（IR）已在临床治疗中应用了很多年，放射敏感性对治疗至关重要。细胞的放射敏感性受到许多环境因素的影响，例如缺氧和温度。在许多研究中，高渗透压作为一种常见的环境因素已被证明与细胞的存活和凋亡有关。因此，我们研究了高渗透压对细胞放射敏感性的影响。方法：我们检查了照射过的L-O2细胞的L-O2细胞的存活率和存活率，并通过流式细胞术检测了其对AHH-1细胞的影响，以研究短期高渗预处理对细胞放射敏感性的影响。彗星试验用于评估DNA链断裂。然后通过蛋白质印迹法检测Akt1和通过CYSY-TT增加调节体积的过程参与了该机理。结果：我们证明了对细胞进行短期高渗预处理可以降低其放射敏感性。进一步的研究表明，短期高渗状态可能会引起调节体积增加（RVI），从而激活Akt1并使IκB-α变性。该过程与细胞放射敏感性降低有关。最后，我们使用了氟芬那酸（FFA），一种阳离子通道（HICC）的阻滞剂来居住RVI，因此居住了高渗透压对照射细胞的保护作用。结论：短期高渗预处理可降低RVI和激活Akt1后的细胞放射敏感性。

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