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首页> 外文期刊>Cellular Physiology and Biochemistry >Silencing of ADIPOQ Efficiently Suppresses Preadipocyte Differentiation in Porcine
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Silencing of ADIPOQ Efficiently Suppresses Preadipocyte Differentiation in Porcine

机译：沉默ADIPOQ有效抑制猪的前脂肪细胞分化。

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biAims/i/b Our study aims to characterize the functions of the ADIPOQ gene in the process of fat deposition of pigs, thereby providing a basis for the use of this gene as a molecular marker for pork quality. biMethods/i/b We used healthy Junmu1 piglets less than 7 days of age to establish an iin vitro/i culture system for porcine preadipocytes. Chemically synthesized short hairpin RNAs (shRNA) were transfected into porcine preadipocytes to silence the expression of the ADIPOQ gene. We monitored preadipocyte differentiation and determined the levels of the adipocyte differentiation transcription factors lipoprotein lipase (LPL), peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid binding protein (AP2) mRNAs to investigate the effects of ADIPOQ on the differentiation of porcine preadipocytes. biResults/i/b After transfection, the mRNA and protein levels of the ADIPOQ gene were significantly decreased (P 0.01), the number of lipid droplets in the adipocytes was significantly reduced, the OD values reflecting the fat content were significantly decreased (P 0.01), and the levels of LPL, PPARγ and AP2 were significantly reduced (P 0.01). biConclusions/i/b These results suggest that interference with ADIPOQ gene expression can inhibit the differentiation of porcine preadipocytes.
机译：目标 我们的研究旨在表征ADIPOQ基因在猪脂肪沉积过程中的功能，从而为将该基因用作猪脂肪分子标记提供基础。猪肉品质。 方法 我们使用年龄小于7天的健康Junmu1仔猪建立了用于猪前脂肪细胞的体外培养系统。化学合成的短发夹RNA（shRNA）被转染到猪前脂肪细胞中，以沉默ADIPOQ基因的表达。我们监测脂肪前细胞的分化，并确定脂肪细胞分化转录因子脂蛋白脂酶（LPL），过氧化物酶体增殖物激活受体的水平。 （PPAR＆＃947;）和脂肪细胞脂肪酸结合蛋白（AP2）mRNA，以研究ADIPOQ对猪前脂肪细胞分化的影响。 结果 转染后，ADIPOQ基因的mRNA和蛋白水平显着下降（P <0.01），脂肪细胞中的脂滴数量明显减少，OD反映脂肪含量的数值显着下降（P <0.01），LPL，PPAR＆＃947的水平;和AP2显着降低（P <0.01）。 结论 这些结果表明，干扰ADIPOQ基因表达可以抑制猪前脂肪细胞的分化。

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