...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >Nuclear Transport of Wilms′ Tumour Protein Wt1 Involves Importins α and β
【24h】

Nuclear Transport of Wilms′ Tumour Protein Wt1 Involves Importins α and β

机译：Wilms肿瘤蛋白Wt1的核转运涉及Importinsα和β

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background/Aims Wilms′ tumour protein, Wt1, is a zinc finger molecule, which is required for normal embryonic development. Mutations of the iWT1/i gene can give rise to childhood cancer of the kidneys. Different Wt1 isoforms exist, which function either as transcription factors or have a presumed role in mRNA processing. Previous studies suggested that Wt1 undergoes nucleocytoplasmic shuttling, and icytoplasmic/i Wt1 was higher in malignant than in normal cells. The aim of this study was to analyse the molecular pathways along which Wt1 shuttles between the cytoplasm and nucleus. Methods Interaction of Wt1 protein with various importin α subtypes and importin β was assessed in pull-down assays and co-immunoprecipitation experiments. Nuclear localisation signals (NLS) were identified by combining site-directed mutagenesis with subcellular immunodetection of the transfected Wt1 variants. Results Wt1(+/-KTS) proteins were found to interact with importin α1 and importin β iin vitro/i and in living cells iin vivo/i. A NLS that was necessary and sufficient for nuclear import could be mapped to the third Wt1 zinc finger. Mutation of this NLS strongly weakened binding of Wt1 to importins. Conclusion Nuclear translocation of Wilms′ tumour protein involves importins α and β, and a NLS in the third zinc finger.
机译：背景/目的威尔姆斯的肿瘤蛋白Wt1是锌指分子，是正常胚胎发育所必需的。 WT1 基因的突变会引起儿童肾脏癌。存在不同的Wt1同工型，它们充当转录因子或在mRNA加工中具有假定的作用。先前的研究表明，Wt1经历了核质穿梭，并且胞质性 Wt1在恶性肿瘤中比在正常细胞中高。这项研究的目的是分析Wt1在细胞质和细胞核之间穿梭的分子途径。方法通过下拉法和免疫共沉淀实验评估Wt1蛋白与各种importinα亚型和importinβ的相互作用。通过将定点诱变与转染的Wt1变体的亚细胞免疫检测相结合来鉴定核定位信号（NLS）。结果发现Wt1（+/- KTS）蛋白在体外和在活细胞中均与importinα1和importinβ相互作用。可以将用于核进口的必要和充分的NLS映射到第三个Wt1锌指。此NLS的突变强烈削弱了Wt1与importins的结合。结论Wilms肿瘤蛋白的核易位涉及输入蛋白α和β，以及第三个锌指中的NLS。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号