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Effect of Oxygen Tension on the Amino Acid Utilisation of Human Embryonic Stem Cells

机译：氧张力对人胚胎干细胞氨基酸利用的影响

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biBackground/Aims /i/bHuman embryonic stem cells (hESCs) are a potential source of cells for treatment of many degenerative diseases, but in culture have a propensity to spontaneously differentiate, possibly due to suboptimal conditions. Culture at low oxygen tensions improves hESC maintenance and regulates carbohydrate metabolism. Hence, a greater understanding of the nutrient requirements of hESCs will allow production of more appropriate culture media. This study aims to investigate the effect of environmental oxygen tension on the amino acid metabolism of hESCs. biMethods /i/bThe production or depletion of amino acids by hESCs cultured at 5% or 20% oxygen in the presence or absence of FGF2 was measured by reversephase HPLC. biResults /i/bAtmospheric oxygen, or removal of FGF2 from hESCs cultured at 5% oxygen, perturbed the uptake or release of individual amino acids and the total amino acid turnover compared to hESCs cultured at 5% oxygen. In particular, serine uptake was reduced at 20% oxygen and by removal of FGF2. biConclusions /i/bHighly pluripotent hESCs, cultured at 5% oxygen, demonstrate a greater amino acid turnover than hESCs cultured at 20% oxygen, or without FGF2. These data suggest that amino acid turnover could be used as a measure of the self-renewal capacity of hESCs.
机译：背景/目标 人类胚胎干细胞（hESCs）是治疗许多退行性疾病的潜在细胞来源，但在培养中具有自发分化的倾向，可能是由于次优条件。低氧张力下的培养可改善hESC的维持并调节碳水化合物的代谢。因此，对hESCs营养需求的更多了解将允许生产更合适的培养基。这项研究旨在调查环境氧紧张对hESCs氨基酸代谢的影响。 方法 通过反相HPLC测定在FGF2存在或不存在下在5％或20％氧气下培养的hESC产生或消耗氨基酸的情况。 结果 大气氧，或从5％氧培养的hESC中去除FGF2，与在hESC培养的hESC相比，干扰了单个氨基酸的吸收或释放以及总氨基酸周转率5％的氧气。尤其是，在20％的氧气下以及通过去除FGF2，丝氨酸的摄取减少了。 结论 高度多能的hESC（在5％氧气条件下培养）比在20％氧气或不添加FGF2的hESCs中具有更高的氨基酸转换率。这些数据表明，氨基酸更新可以用作hESCs自我更新能力的量度。

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