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首页> 外文期刊>Cellular Physiology and Biochemistry >Histamine Induces Bovine Rumen Epithelial Cell Inflammatory Response via NF-?oB Pathway
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Histamine Induces Bovine Rumen Epithelial Cell Inflammatory Response via NF-?oB Pathway

机译：组胺通过NF-κB途径诱导牛瘤胃上皮细胞炎症反应

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>Background/Aims: Subacute ruminal acidosis (SARA) is a common disease in high-producing lactating cows. Rumenitis is the initial insult of SARA and is associated with the high concentrations of histamine produced in the rumen of dairy cows during SARA. However, the exact mechanism remains unclear. The objective of the current study is to investigate whether histamine induces inflammation of rumen epithelial cells and the underlying mechanism of this process. Methods: Bovine rumen epithelial cells were cultured and treated with different concentrations of histamine and pyrrolidine dithiocarbamate (PDTC, an NF-?oB inhibitor) cultured in different pH medium (pH 7.2 or 5.5). qRT-PCR, Western-blotting, ELISA and immunocytofluorescence were used to evaluate whether histamine activated the NF-?oB pathway and inflammatory cytokines. Results: The results showed that histamine significantly increased the activity of IKK ?2 and the phosphorylation levels of I?oB ?±, as well as upregulated the mRNA and protein expression levels of NF-?oB p65 in the rumen epithelial cells cultured in neutral (pH=7.2) and acidic (pH=5.5) medium. Furthermore, histamine treatment also significantly increased the transcriptional activity of NF-?oB p65. High expression and transcriptional activity of NF-?oB p65 significantly increased the mRNA expressions and concentrations of inflammatory cytokines, tumor necrosis factor alpha (TNF-?±), interleukin 6 (IL-6) and interleukin 1 beta (IL-1?2), thereby inducing the inflammatory response in bovine rumen epithelial cells. However, inhibition of NF-?oB p65 by PDTC significantly decreased the expressions and concentrations of the inflammatory cytokines induced by histamine in the rumen epithelial cells cultured in the neutral and acidic medium. Conclusion: The present data indicate that histamine induces the inflammatory response of bovine rumen epithelial cells through the NF-?oB pathway.
机译：> 背景/目标： 亚急性瘤胃酸中毒（SARA）是高产泌乳母牛的常见疾病。瘤胃炎是SARA的最初侵害，与SARA期间奶牛瘤胃中产生的高浓度组胺有关。但是，确切的机制仍不清楚。本研究的目的是研究组胺是否诱导瘤胃上皮细胞发炎以及该过程的潜在机制。 方法： 培养牛瘤胃上皮细胞，并用不同浓度的组胺和吡咯烷二硫代氨基甲酸酯（PDTC，一种NF-κB抑制剂）在不同pH值的培养基（pH 7.2）中培养或5.5）。使用qRT-PCR，Western印迹，ELISA和免疫细胞荧光来评估组胺是否激活了NF-κB通路和炎性细胞因子。 结果： 结果表明，组胺可显着提高IKKβ2的活性和IκoBα±的磷酸化水平，并上调mRNA和蛋白质表达水平中性（pH = 7.2）和酸性（pH = 5.5）培养基中培养的瘤胃上皮细胞中NF-κBB p65的表达。此外，组胺处理还显着提高了NF-κBp65的转录活性。 NF-ΔoBp65的高表达和转录活性显着增加了炎症细胞因子，肿瘤坏死因子α（TNF-α±），白介素6（IL-6）和白介素1 beta（IL-1？2）的mRNA表达和浓度。 ），从而在牛瘤胃上皮细胞中诱导炎症反应。然而，PDTC对NF-κBp65的抑制显着降低了在中性和酸性介质中培养的瘤胃上皮细胞中由组胺诱导的炎性细胞因子的表达和浓度。 结论： 目前的数据表明，组胺通过NF-κB途径诱导牛瘤胃上皮细胞的炎症反应。

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