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Tissue Distribution and Regulation of the Small Sar1b GTPase in Mice

机译：小鼠Sar1b GTPase的组织分布和调控

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biBackground/Aims /i/bSar1b GTPase (Sar1b) represents an obligatory component of COPII vesicles that bud from the endoplasmic reticulum to transport proteins to the Golgi apparatus. Its genetic mutations lead to a severe disorder known as chylomicron retention disease. Despite growing knowledge on Sar1b, little is known about it tissue distribution and regulation, which constitute the aims of the present study. We aimed to determine the Sar1b tissue distribution and modulation by a high-fat diet and gene forcing using transgenic mice in comparison to wild-type mice. biMethods/i/bbi /i/bThe expression pattern of Sar1b was studied in different organs of wild-type and iSar1b/i transgenic mice by qRT-PCR and Western blot. The effect of transgenesis and insulin resistance induced by a 12-week high-fat diet on iSar1b/i gene expression was also assessed by qRT-PCR. biResults /i/bEvaluation of iSar1b/i mRNA revealed the skeletal muscle as the tissue with the highest iSar1b/i expression, followed by the heart and liver, the organs composing the digestive tract, the brain and finally the lung and the adipose tissue. Sar1b protein expression levels follow a similar pattern among the organs, except for its lower expression in the heart. While the high-fat diet did not exert any significant alterations, Sar1b transgenic mice displayed higher gene expression in the liver, ileum, jejunum, proximal and distal colon compared to wild-type mice. biConclusion /i/bOur study supports the importance of Sar1b in organs involved in lipid transport and/or calcium trafficking such as the liver, intestine, skeletal muscle and heart.
机译：背景/目标 Sar1b GTPase（Sar1b）代表COPII囊泡的必需成分，该囊泡从内质网发芽以将蛋白质转运至高尔基体。它的基因突变导致一种严重的疾病，称为乳糜微粒保留病。尽管对Sar1b的了解不断增长，但对它的组织分布和调节知之甚少，这些构成了本研究的目的。我们的目的是通过与野生型小鼠相比，使用转基因小鼠通过高脂饮食和基因强迫来确定Sar1b组织的分布和调控。 方法 研究了Sar1b在野生型和 Sar1b的不同器官中的表达模式基因表达的影响。 结果 对 Sar1b mRNA的评估表明，骨骼肌是 Sar1b 表达最高的组织，其次是心脏以及肝脏，组成消化道的器官，大脑，最后是肺和脂肪组织。 Sar1b蛋白的表达水平在器官之间遵循相似的模式，但在心脏中的表达较低。尽管高脂饮食没有任何明显的改变，但与野生型小鼠相比，Sar1b转基因小鼠在肝脏，回肠，空肠，近端和远端结肠中显示出更高的基因表达。 结论 我们的研究支持Sar1b在参与脂质转运和/或钙转运的器官（如肝脏，肠，骨骼肌和心脏）中的重要性。

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