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首页> 外文期刊>Cellular Physiology and Biochemistry >Effects of Glucose Restriction on Replicative Senescence of Human Diploid Fibroblasts IMR-90
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Effects of Glucose Restriction on Replicative Senescence of Human Diploid Fibroblasts IMR-90

机译：葡萄糖限制对人二倍体成纤维细胞IMR-90复制衰老的影响

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Background/Aims:. The aim of this study was to elucidate the effects of glucose restriction (GR) on cell replicative senescence in vitro by human diploid fibroblasts IMR-90. Methods: IMR-90 cells were cultured under 40, 60% GR or high glucose medium and biomarkers of cell senescence were compared with cells cultured in normal glucose medium (5.5 mM glucose). The impact of different concentrations of glucose and initial passages on cell replicative senescence were assessed by cell survival days, cumulative population doublings (PD), cell proliferation rate (CPR) and SA-β-gal site-stain. Results: When compared with control cells, mean survival days and lifespan of IMR-90 were increased 16.7% and 11.4% by 40% GR (3.3 mM glucose). However, mean survival days and lifespan of IMR-90 were decreased 31.0% and 26.9% by HG treatment (25.0 mM glucose). The effects on survival days of IMR-90 were associated not only with different glucose concentrations but also with initial passages. The CPR of IMR-90 could be retarded by GR culture and this effect was especially associated with GR degree. It was 87% positive cells of SA-β-gal in aging stages and more slim and fibrous cells were observed in 40% GR group than NG group onset from 26 PD. Conclusion: Mean survival days and lifespan of human diploid fibroblasts IMR-90 were extended by glucose restriction. The higher GR levels, the earlier onset of GR, the larger benefits on extending survival days of IMR-90 could be observed. Slowing down cell proliferation by GR increased the number of cell survival days, an effect associated with GR levels. High glucose induced premature senescence of IMR-90 when started from any passages.
机译：背景/目标：这项研究的目的是阐明人二倍体成纤维细胞IMR-90在体外对葡萄糖限制（GR）的影响对细胞复制衰老的影响。方法：将IMR-90细胞在40％，60％GR或高葡萄糖培养基中培养，并将其衰老的生物标志物与在正常葡萄糖培养基（5.5 mM葡萄糖）中培养的细胞进行比较。通过细胞存活天数，累积群体倍增（PD），细胞增殖率（CPR）和SA-β-gal位点染色评估了不同浓度的葡萄糖和初始传代对细胞复制衰老的影响。结果：与对照细胞相比，IMR-90的平均存活天数和寿命通过40％GR（3.3 mM葡萄糖）增加了16.7％和11.4％。但是，通过HG治疗（25.0 mM葡萄糖），IMR-90的平均存活天数和寿命分别降低了31.0％和26.9％。 IMR-90对存活天数的影响不仅与不同的葡萄糖浓度有关，而且与初始传代有关。 GR培养可以抑制IMR-90的CPR，这一作用尤其与GR程度有关。从26 PD开始，在衰老阶段SA-β-gal的阳性细胞为87％，在40％的GR组中观察到比NG组更多的纤细和纤维细胞。结论：葡萄糖限制可延长人二倍体成纤维细胞IMR-90的平均存活天数和寿命。 GR水平越高，GR发作越早，可以观察到延长IMR-90生存期的更大益处。通过GR减慢细胞增殖，增加了细胞存活天数，这与GR水平有关。从任何传代开始，高葡萄糖都会引起IMR-90的过早衰老。

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