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首页> 外文期刊>Cellular Physiology and Biochemistry >Stimulation of Erythrocyte Cell Membrane Scrambling by Gedunin
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Stimulation of Erythrocyte Cell Membrane Scrambling by Gedunin

机译：葛根素刺激红细胞膜的扰动

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biBackground/Aims /i/bGedunin, an inhibitor of heat shock protein HSP90, triggers apoptosis of tumor cells and is thus effective against malignancy. Moreover, the drug has antimalarial potency. In analogy to apoptosis of nucleated cells, erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and by phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Casup2+/sup-activity ([Casup2+/sup]subi/sub). The present study explored whether gedunin stimulates eryptosis. biMethods /i/bForward scatter was determined to estimate cell volume, annexin V binding to identify phosphatidylserine-exposing erythrocytes, hemoglobin release to depict hemolysis, and Fluo3-fluorescence to quantify [Casup2+/sup]subi/sub. biResults /i/bA 48 h exposure of human erythrocytes to gedunin significantly increased [Casup2+/sup]subi/sub (12 µM), significantly decreased forward scatter (24 µM) and significantly increased annexin-V-binding (12 µM). The effect of gedunin (24 µM) on annexin-V-binding was virtually abrogated by removal of extracellular Casup2+/sup. biConclusion /i/bGedunin stimulates suicidal erythrocyte death or eryptosis, an effect mainly if not exclusively due to stimulation of Casup2+/sup entry.
机译：背景/目的 Gedunin是热休克蛋白HSP90的抑制剂，可触发肿瘤细胞的凋亡，因此对恶性肿瘤有效。此外，该药物具有抗疟疾的功效。类似于有核细胞的凋亡，红细胞可能会进入自杀死亡或隐匿状态，其特征是细胞萎缩和磷脂酰丝氨酸易位至红细胞表面。胞浆中Ca 2 + -（[Ca 2 + ] i ）活性的增加可能触发了加密。本研究探讨了葛根素是否刺激隐匿性。 方法 确定前向散射以估计细胞体积，膜联蛋白V结合以鉴定暴露磷脂酰丝氨酸的红细胞，血红蛋白释放以描述溶血，而Fluo3-荧光定量[Ca 2 + ] i 。 结果 人类红细胞暴露于gedunin中48小时会显着增加[Ca 2 + ] i （12 µM） ，显着降低前向散射（24 µM），并显着增加膜联蛋白V结合（12 µM）。去除细胞外Ca 2 + 实际上消除了葛根素（24 µM）对膜联蛋白V结合的影响。 结论 Gedunin刺激自杀性红细胞死亡或隐匿，主要是（而不是完全）由于刺激Ca 2 + 进入而引起的。

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