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首页> 外文期刊>Cellular Physiology and Biochemistry >Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients
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Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients

机译：原发性胆源性胆管炎患者血浆中环状RNA的芯片表达谱

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>Background/Aims: Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases, including autoimmune diseases. However, their role in primary biliary cholangitis (PBC) remains unclear. Here, we aimed to determine the circRNA expression profile in plasma from PBC patients and further explore the value of circRNA in diagnosing PBC. Methods: CircRNA microarrays were used to determine circRNA expression profiles in plasma samples from 6 PBC patients and 6 healthy controls. Statistical analyses identified differentially expressed circRNAs, and these circRNAs were verified by qRT-PCR in 29 PBC patients and 30 healthy controls. MicroRNA (miRNA) target prediction software identified putative miRNA response elements (MREs), which were used to construct a map of circRNA-miRNA interactions for the differentially expressed circRNAs. Results: Our results indicated that there were 18 up-regulated and 4 down-regulated circular RNAs in the plasma from PBC patients compared with that from healthy individuals. Among the differentially expressed circRNAs, hsa_circ_402458 (P=0.0033), hsa_circ_087631 and hsa_circ_406329 (P=0.0185) were up-regulated, and hsa_circ_407176 (P=0.0066) and hsa_circ_082319 were down-regulated in the PBC group versus the healthy group as demonstrated by qRT-PCR. In particular, hsa_circ_402458 was significantly higher in PBC patients not receiving UDCA treatment than in PBC patients receiving UDCA treatment (P=0.0338). The area under the receiver operating characteristic curve for hsa_circ_402458 for diagnosing PBC was 0.710 (P=0.005). For hsa_circ_402458, two putative miRNA targets, hsa-miR-522-3p and hsa-miR-943, were predicted. Conclusions: circRNA dysregulation may play a role in PBC pathogenesis, and hsa_circ_402458 shows promise as a candidate biomarker for PBC.
机译：> 背景/目标： 环状RNA（circRNA）在多种疾病（包括自身免疫性疾病）的发生中起着至关重要的作用。然而，它们在原发性胆源性胆管炎（PBC）中的作用仍不清楚。在这里，我们旨在确定circRNA在PBC患者血浆中的表达谱，并进一步探讨circRNA在PBC诊断中的价值。 方法： CircRNA微阵列用于确定6例PBC患者和6例健康对照者血浆样品中的circRNA表达谱。统计分析确定了差异表达的circRNA，并通过qRT-PCR在29例PBC患者和30例健康对照中验证了这些circRNA。 MicroRNA（miRNA）目标预测软件识别了推定的miRNA反应元件（MRE），该元件用于构建差异表达circRNA的circRNA-miRNA相互作用图。 结果： 我们的结果表明，与健康人相比，PBC患者血浆中有18个上调的环状RNA和4个下调的环状RNA。在差异表达的circRNA中，hsa_circ_402458（ P = 0.0033），hsa_circ_087631和hsa_circ_406329（ P = 0.0185）上调，hsa_circ_407176（ P > = 0.0066）和hsa_circ_082319在pBC组中相对于健康组下调，如qRT-PCR所示。特别是，未接受UDCA治疗的PBC患者的hsa_circ_402458明显高于接受UDCA治疗的PBC患者（ P = 0.0338）。用于诊断PBC的hsa_circ_402458的接收器工作特性曲线下的面积为0.710（ P = 0.005）。对于hsa_circ_402458，预测了两个推定的miRNA靶标，即hsa-miR-522-3p和hsa-miR-943。 结论： circRNA失调可能在PBC发病机理中起作用，hsa_circ_402458显示出有望成为PBC的候选生物标志物。

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