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首页> 外文期刊>Cellular Physiology and Biochemistry >SUMO-1 Gene Silencing Inhibits Proliferation and Promotes Apoptosis of Human Gastric Cancer SGC-7901 Cells
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SUMO-1 Gene Silencing Inhibits Proliferation and Promotes Apoptosis of Human Gastric Cancer SGC-7901 Cells

机译：SUMO-1基因沉默抑制人胃癌SGC-7901细胞的增殖并促进其凋亡

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>Background: It has been reported that blocking small ubiquitin-like modifier (SUMO) conjugation by silencing SUMO gene remarkably decreased tumor growth in vivo. However, few studies have examined the relationship between SUMO gene silencing and gastric cancer (GC). The study aims to explore the effects of SUMO-1 gene silencing on GC cell proliferation and apoptosis. Methods: GC cells were cultured and divided into 5 groups: the blank group (without any transfection or treatment), the empty vector group (transfected with empty vector), the shRNA-SUMO-1-1 group (transfected with shRNA-SUMO-1-1 plasmid), the shRNA-SUMO-1-2 group (transfected with shRNA-SUMO-1-2 plasmid), and the shRNA-SUMO-1-3 group (transfected with shRNA-SUMO-1-3 plasmid). Cell Counting Kit-8 (CCK-8) assay was performed to examine cell proliferation. Annexin V/PI staining combined with flow cytometry were used to detect cell apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were employed to measure the mRNA and protein expressions of SUMO-1, P53, Bcl-2 and c-myc, respectively. Results: SUMO-1 mRNA and protein expressions were decreased after transfecting with shRNA-SUMO-1. Compared with the blank group, the shRNA-SUMO-1-1 group presented a remarkable decreased proliferation of SGC-7901 cells. Significant increase in cell apoptosis rate was observed. Bcl-2, c-myc and P53 expressions were declined after transfecting with shRNA-SUMO plasmid. Conclusion: Our study provided evidence that SUMO-1 gene silencing could decrease proliferation and promote apoptosis in GC cells.
机译：> 背景： 据报道，通过沉默 SUMO 基因来阻断小的泛素样修饰物（SUMO）偶联显着降低了肿瘤的生长< i>体内。但是，很少有研究检查SUMO基因沉默与胃癌（GC）之间的关系。该研究旨在探讨 SUMO-1 基因沉默对GC细胞增殖和凋亡的影响。 方法： 将GC细胞培养并分为5组：空白组（不进行任何转染或处理），空载体组（用空载体转染），shRNA -SUMO-1-1组（已转染shRNA-SUMO-1-1质粒），shRNA-SUMO-1-2组（已转染shRNA-SUMO-1-2质粒）和shRNA-SUMO-1- 3组（用shRNA-SUMO-1-3质粒转染）。进行细胞计数试剂盒8（CCK-8）分析以检查细胞增殖。 Annexin V / PI染色结合流式细胞仪检测细胞凋亡。定量实时聚合酶链反应（qRT-PCR）和蛋白质印迹分别用于测量SUMO-1，P53，Bcl-2和c-myc的mRNA和蛋白质表达。 结果： SUMO-1 shRNA-SUMO-1转染后mRNA和蛋白质表达降低。与空白组相比，shRNA-SUMO-1-1组的SGC-7901细胞增殖明显降低。观察到细胞凋亡率显着增加。 shRNA-SUMO质粒转染后，Bcl-2，c-myc和P53表达下降。 结论： 我们的研究提供了证据，证明 SUMO-1 基因沉默可以降低GC细胞的增殖并促进其凋亡。

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