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首页> 外文期刊>Cellular Physiology and Biochemistry >High Insulin Concentrations Repress Insulin Receptor Gene Expression in Calf Hepatocytes Cultured in Vitro
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High Insulin Concentrations Repress Insulin Receptor Gene Expression in Calf Hepatocytes Cultured in Vitro

机译：高胰岛素浓度抑制体外培养的小牛肝细胞中胰岛素受体基因的表达

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The major role of insulin and the insulin receptor (InsR) in the liver is to mediate glucose uptake into hepatocytes to synthesize glycogen and to maintain blood glucose homeostasis. In this study, we investigated the effects of high insulin concentrations on InsR gene expression in calf hepatocytes cultured iin vitro/i. After the cells were cultured for 72 h, insulin was added to the culture solution at final concentrations of 0, 1, 10, 100 or 1000 nM. InsR mRNA expression was determined by semi-quantitative RT-PCR. The results showed that InsR mRNA expression in hepatocytes, adjusted for β-actin expression, decreased dose dependently with increasing insulin concentration. InsR mRNA expression was similar at 1 and 10 nM insulin, but was significantly lower than that in the control. InsR expression was similar at 100 and 1000 nM insulin, but was significantly lower than that in the control, 1 and 10 nM insulin groups. These data suggest that high concentrations of insulin significantly repress InsR mRNA expression in calf hepatocytes, and this inhibition occurs in a dose-dependent manner. Further studies are needed to investigate the mechanisms underlying these effects of insulin.
机译：肝脏中胰岛素和胰岛素受体（InsR）的主要作用是介导葡萄糖摄取进入肝细胞以合成糖原并维持血糖稳态。在这项研究中，我们研究了高胰岛素浓度对体外培养的小牛肝细胞InsR基因表达的影响。将细胞培养72小时后，将胰岛素以终浓度0、1、10、100或1000nM添加到培养溶液中。通过半定量RT-PCR确定InsR mRNA表达。结果表明，调节β-肌动蛋白表达后，肝细胞中InsR mRNA的表达随胰岛素浓度的增加而剂量依赖性地降低。 InsR mRNA表达在1和10 nM胰岛素时相似，但显着低于对照。在100和1000 nM胰岛素时，InsR表达相似，但明显低于对照组，1和10 nM胰岛素组。这些数据表明，高浓度的胰岛素可显着抑制小牛肝细胞中InsR mRNA的表达，并且这种抑制作用呈剂量依赖性。需要进一步的研究来研究胰岛素这些作用的潜在机制。

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