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首页> 外文期刊>Cellular Physiology and Biochemistry >Downregulative Effects of Nitric Oxide on Oocyte Fertilization and Embryo Development: Possible Roles of Nitric Oxide in the Pathogenesis of Endometriosis-associated Infertility
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Downregulative Effects of Nitric Oxide on Oocyte Fertilization and Embryo Development: Possible Roles of Nitric Oxide in the Pathogenesis of Endometriosis-associated Infertility

机译：一氧化氮对卵母细胞受精和胚胎发育的下调作用：一氧化氮在子宫内膜异位症相关不孕症发病机制中的可能作用

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Aims To investigate the effects of elevated nitric oxide (NO) levels in peritoneal fluids (PF) on oocyte fertilization and pre-implantation embryo development, and the relation of those effects to endometriosis-associated infertility. Methods PF from women undergoing laparoscopy for infertility of minor endometriosis, tubal blockage and operation for tubal ligation was aspired at the pouch of the cul-de-sac during surgery. Oocytes and embryos of adult ICR mice were cultured iin vitro/i with or without endometriotic PF. The fertilization rate of oocyte and the cleavage rate of 2-cell embryos were examined. Also, the clinical indexes of IVF-ET of women with minor endometriosis and tubal infertility were analyzed. Results Oocyte fertilization rate of endometriotic women with IVF-ET treatment was significantly lower than that of tubal block women. The dose-related adverse effects of endometriotic PF and SNP (NO donor) in culture medium on oocyte fertilization and embryos development were confirmed. Conclusion Increased NO levels in PF play an important role in mediating the effects of endometriotic PF on oocyte fertilization and embryo development. IVF might serve as an alternative treatment for endometriosis-associated infertility.
机译：目的探讨腹膜液（PF）中一氧化氮（NO）水平升高对卵母细胞受精和植入前胚胎发育的影响，以及这些影响与子宫内膜异位症相关的不育症的关系。方法在手术期间，将因腹膜镜检查的女性因小内异症，输卵管阻塞和输卵管结扎术不孕而进行的PF抽吸至小路囊袋。成年ICR小鼠的卵母细胞和胚胎在有或没有子宫内膜异位PF的条件下进行体外培养。检查卵母细胞的受精率和2-细胞胚胎的卵裂率。此外，分析了患有轻微子宫内膜异位和输卵管不育的女性的IVF-ET的临床指标。结果IVF-ET治疗子宫内膜异位妇女的卵母细胞受精率明显低于输卵管阻塞妇女。证实了子宫内膜异位PF和SNP（NO供体）在剂量上对卵母细胞受精和胚胎发育的不利影响。结论PF中NO水平的升高在介导子宫内膜异位PF对卵子受精和胚胎发育的影响中起着重要作用。 IVF可以作为子宫内膜异位相关性不孕症的替代疗法。

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