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PSMA7 Directly Interacts with NOD1 and Regulates its Function

机译：PSMA7直接与NOD1相互作用并调节其功能

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biBackground/Aims /i/bRecent reports showed that proteasome subunit alpha type-7 (PSMA7) was overexpressed in colorectal cancer. To investigate the mechanism of PSMA7 in promotion of colorectal cancer, we screened for its interaction partners. biMethods and Results /i/bThis study found that PSMA7 associated with nucleotide-binding oligomerization domain-containing protein 1 (NOD1) by yeast two-hybrid screening, co-immunoprecipitation (IP), and GST-pull down assay. As shown by Western blotting and ubiquitin assay, PSMA7 downregulated the expression of NOD1 in a proteasome-dependent manner. Overexpression of PSMA7 in HCT116 cells resulted in an inhibition of NOD1-mediated apoptosis and NF-κB activation, whereas knockdown of PSMA7 by RNA interference enhanced NOD1 activity. biConclusion /i/bOur data suggest that PSMA7 is a negative regulator of the NOD1, and may promote tumor growth by its inhibitory role on NOD1.
机译：背景/目标 最近的报告显示，在大肠癌中蛋白酶体亚基α7型（PSMA7）过表达。为了研究PSMA7促进结直肠癌的机制，我们筛选了其相互作用伙伴。 方法和结果 本研究发现，通过酵母双杂交筛选，共免疫沉淀（IP），PSMA7与含有核苷酸结合低聚域的蛋白1（NOD1）相关，和GST下拉测定法。如蛋白质印迹和泛素测定所示，PSMA7以蛋白酶体依赖性方式下调了NOD1的表达。 HCT116细胞中PSMA7的过度表达导致NOD1介导的凋亡和NF-＆＃954; B激活受到抑制，而RNA干扰抑制PSMA7则增强了NOD1活性。 结论 我们的数据表明PSMA7是NOD1的负调节剂，并可能通过其对NOD1的抑制作用促进肿瘤生长。

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