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首页> 外文期刊>Cellular Physiology and Biochemistry >MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4
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MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

机译：MicroRNA-212通过下调Sox4抑制骨肉瘤细胞的增殖和侵袭

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biBackground /i/bMultiple MicroRNAs (miRNAs) have been identified in the development and progression of osteosarcoma. However, the expression and roles of miR-212 in osteosarcoma remain largely undefined. biMethods /i/bReal-time PCR assays were used to detect the expression of miR-212 in human osteosarcoma tissues. MiR-212 mimics were introduced into MG63 and U2OS cells. Bioinformatic prediction was used to identify the potential targets of miR-212. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-212. biResults /i/bmiR-212 was significantly down-regulated in human osteosarcoma tissues, compared with adjacent normal tissues. Introduction of miR-212 mimics into MG63 and U2OS cells inhibited cell proliferation and invasion. Besides, miR-212 overexpression could also inhibit tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the sex-determining region Y-box 4 (Sox4) is a target gene of miR-212. Sox4 inhibition phenocopied the roles of miR-212, while restored expression of Sox4 dampened miR-212-mediated suppression of tumor progression. biConclusion /i/bThe miR-212/Sox4 interaction plays an important role of in the osteosarcoma progression.
机译：背景 在骨肉瘤的发展和进程中已经发现了多种MicroRNA（miRNA）。但是，miR-212在骨肉瘤中的表达和作用仍不清楚。 方法 实时荧光定量PCR检测人类骨肉瘤组织中miR-212的表达。将MiR-212模拟物引入MG63和U2OS细胞。生物信息学预测被用于识别miR-212的潜在目标。蛋白表达分析，荧光素酶测定和拯救测定用于确认miR-212的底物。 结果 与邻近的正常组织相比，miR-212在人骨肉瘤组织中显着下调。将miR-212模拟物引入MG63和U2OS细胞可抑制细胞增殖和侵袭。此外，miR-212过表达还可以抑制裸鼠体内的肿瘤生长。此外，生物信息学预测表明，性别决定区域Y-box 4（Sox4）是miR-212的靶基因。 Sox4抑制表型复制了miR-212的作用，而恢复的Sox4表达抑制了miR-212介导的肿瘤进展抑制作用。 结论 miR-212 / Sox4相互作用在骨肉瘤进展中起重要作用。

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