• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants
【24h】

Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

机译：降低软骨细胞密度的软骨引发增强了马同种异体MSC的软骨粘附-在180种外植体器官培养研究中的加载敏感现象

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Clinical results of regenerative treatments for osteoarthritis are becoming increasingly significant. However, several questions remain unanswered concerning mesenchymal stem cell (MSC) adhesion and incorporation into cartilage. Methods: To this end, peripheral blood (PB) MSCs were chondrogenically induced and/or stimulated with pulsed electromagnetic fields (PEMFs) for a brief period of time just sufficient to prime differentiation. In an organ culture study, PKH26 labelled MSCs were added at two different cell densities (0.5 x106 vs 1.0 x106). In total, 180 explants of six horses (30 per horse) were divided into five groups: no lesion (i), lesion alone (ii), lesion with na?ve MSCs (iii), lesion with chondrogenically-induced MSCs (iv) and lesion with chondrogenically-induced and PEMF-stimulated MSCs (v). Half of the explants were mechanically loaded and compared with the unloaded equivalents. Within each circumstance, six explants were histologically evaluated at different time points (day 1, 5 and 14). Results: COMP expression was selectively increased by chondrogenic induction (p = 0.0488). PEMF stimulation (1mT for 10 minutes) further augmented COL II expression over induced values (p = 0.0405). On the other hand, MSC markers remained constant over time after induction, indicating a largely predifferentiated state. In the unloaded group, MSCs adhered to the surface in 92.6% of the explants and penetrated into 40.7% of the lesions. On the other hand, physiological loading significantly reduced surface adherence (1.9%) and lesion filling (3.7%) in all the different conditions (p < 0.0001). Remarkably, homogenous cell distribution was characteristic for chondrogenic induced MSCs (+/- PEMFs), whereas clump formation occurred in 39% of uninduced MSC treated cartilage explants. Finally, unloaded explants seeded with a moderately low density of MSCs exhibited greater lesion filling (p = 0.0022) and surface adherence (p = 0.0161) than explants seeded with higher densities of MSCs. In all cases, the overall amount of lesion filling decreased from day 5 to 14 (p = 0.0156). Conclusion: The present study demonstrates that primed chondrogenic induction of MSCs at a lower cell density without loading results in significantly enhanced and homogenous MSC adhesion and incorporation into equine cartilage.
机译：背景：骨关节炎的再生治疗的临床结果变得越来越重要。然而，关于间充质干细胞（MSC）的粘附以及如何结合到软骨中，仍然有几个问题尚待解答。方法：为此，在短时间内用脉冲电磁场​​（PEMF）软骨诱导和/或刺激外周血（PB）MSC，足以引发分化。在一项器官培养研究中，以两种不同的细胞密度（0.5 x10 6 与1.0 x10 6 ）添加了PKH26标记的MSC。总共将180只外植体（六匹马）（每只马30只）分为五组：无病变（i），仅病变（ii），具有幼稚MSC的病变（iii），具有软骨源性MSC的病变（iv）软骨形成和PEMF刺激的MSC引起的病变（v）。机械外植体的一半被机械装载，并与未装载的等效物进行比较。在每种情况下，在不同的时间点（第1、5和14天）对6个外植体进行组织学评估。结果：通过软骨诱导，COMP表达选择性增加（p = 0.0488）。 PEMF刺激（1mT，持续10分钟）进一步使COL II表达超过诱导值（p = 0.0405）。另一方面，MSC标记物在诱导后随时间保持恒定，表明很大程度上是预分化状态。在无负荷组中，MSCs附着在92.6％的外植体表面，并渗透到40.7％的病灶中。另一方面，在所有不同条件下（p <0.0001），生理负荷显着降低了表面粘附（1.9％）和病变填充（3.7％）。值得注意的是，软骨诱导的MSC（+/- PEMF）具有特征性的均匀细胞分布，而39％的未诱导MSC处理的软骨外植体中均发生团块形成。最后，与中等密度的MSC相比，以中等低密度的MSC接种的外植体表现出更大的病灶填充（p = 0.0022）和表面粘附（p = 0.0161）。在所有情况下，病变填充的总量从第5天减少到第14天（p = 0.0156）。结论：本研究表明，在不加负荷的情况下以较低的细胞密度引发MSC的软骨形成诱导，可显着增强均匀的MSC粘附力，并使其融合到马软骨中。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号