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首页> 外文期刊>Cellular Physiology and Biochemistry >Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-?oB and ERK1/2 MAPK Pathways
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Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-?oB and ERK1/2 MAPK Pathways

机译：碧萝ogen对大鼠星形胶质细胞通过NF-κB和ERK1 / 2 MAPK途径缺氧/缺氧所致神经损伤的保护作用。

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>Backgrounds/Aims: Pycnogenol (PYC) is a patented mix of bioflavonoids with potent anti-oxidant and anti-inflammatory properties. In this study, we investigated the effects of PYC on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury in primary rat astrocytes. Methods: The primary rat astrocytes were randomly divided into 6 groups, blank control, OGD/R, OGD/R+PYC (10, 20, 40, and 60 ?μg/mL). The cell activity were detected by MTT and LDH assays, then the levels of oxidant products [malondialdehyde (MDA) and reactive oxygen species (ROS)] , antioxidants [superoxide dismutase (SOD)], mitochondrial membrane potential (MMP) and inflammatory cytokines were detected. In addition, the expression levels of apoptosis-related proteins (Bax, Bcl-2 and Cleaved caspase 3), proinflammatory factors (NF-?oB p65), and p-ERK1/2 were measured by Western blot analysis. Results: The results showed that PYC incubation dose-dependently attenuated cell viability loss, LDH leakage, oxidative stress, inflammatory cytokines accumulation and cell apoptosis caused by OGD/R. Furthermore, PYC pretreatment dose-dependently suppressed OGD/R-induced NF-?oB p65 nuclear translocation, NF-?oB activity and ERK1/2 phosphorylation. Similarly to PYC, NF-?oB inhibitor PDTC and ERK1/2 inhibitor PD098059 dramatically inhibited OGD/R-induced NF-?oB activation, ERK1/2 phosphorylation, and ROS production, as well as TNF-?± secretion. Conclusions: These findings revealed that PYC has neuroprotective effects against OGD/R-induced injury via NF-?oB and ERK1/2 pathways in primary rat astrocytes.
机译：> 背景/目标： 碧萝ogen（PYC）是生物类黄酮的专利混合物，具有有效的抗氧化和抗炎特性。在这项研究中，我们调查了PYC对原代大鼠星形胶质细胞中氧葡萄糖剥夺/复氧（OGD / R）诱导的损伤的影响。 方法： 将原代大鼠星形胶质细胞随机分为6组，分别为空白对照组，OGD / R，OGD / R + PYC（10、20、40和60μg / mL）。通过MTT和LDH测定检测细胞活性，然后测定氧化剂产物[丙二醛（MDA）和活性氧（ROS）]，抗氧化剂[超氧化物歧化酶（SOD）]，线粒体膜电位（MMP）和炎性细胞因子的水平。检测到。另外，通过Western印迹分析测定凋亡相关蛋白（Bax，Bcl-2和Cleaved caspase 3），促炎因子（NF-κBp65）和p-ERK1 / 2的表达水平。 结果： 结果表明，PYC孵育剂量依赖性地降低了OGD / R引起的细胞活力丧失，LDH泄漏，氧化应激，炎性细胞因子蓄积和细胞凋亡。此外，PYC预处理剂量依赖性地抑制了OGD / R诱导的NF-κBp65核移位，NF-κB活性和ERK1 / 2磷酸化。与PYC相似，NF-κB抑制剂PDTC和ERK1 / 2抑制剂PD098059显着抑制OGD / R诱导的NF-κB活化，ERK1 / 2磷酸化，ROS生成以及TNF-α分泌。 结论： 这些发现表明，PYC通过原代大鼠星形胶质细胞中的NF-αoB和ERK1 / 2途径对OGD / R诱导的损伤具有神经保护作用。

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