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首页> 外文期刊>Cellular Physiology and Biochemistry >Cold Exposure Differentially Stimulates Angiogenesis in BAT and WAT of Mice: Implication in Adrenergic Activation
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Cold Exposure Differentially Stimulates Angiogenesis in BAT and WAT of Mice: Implication in Adrenergic Activation

机译：冷暴露差异刺激小鼠BAT和WAT中的血管生成：肾上腺素激活的意义。

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>Background/Aims: To characterize the temporal profile of cold-induced angiogenesis in brown and white adipose tissues of mice in vivo and the temporal changes of angiogenic factors in primary mice brown (BA) and white adipocytes (WA) treated with ?23-adrenoceptor agonist (CL316,243) in vitro. Methods: 8-week old male C57BL/6J mice were individually housed in conventional cages under cold exposure (4?°C) for 1, 2, 3, 4 and 5 days. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous (sWAT) and epididymal white adipose tissues (eWAT) were harvested for immunohistochemical and gene expression analysis. In vitro, primary mice BA and WA treated with or without CL316,243 were harvested for gene expression and protein secretion analysis. Results: A combination of morphological and genetic (Vegfa, Vegfr2, Hif-1?±, Pai1 and Pedf) analyses demonstrated depot-specific angiogenesis in response to cold exposure. Upon CL316,243 treatment, angiogenic factors (Vegfa, Vegfr2, Hif-1?±, Pai1 and Pedf) and secreted protein VEGFA were transiently increased in both BA and WA. Conclusion: Our results show that iBAT is highly responsive to cold-induced angiogenesis that is mainly supported by sWAT with a lesser extent by eWAT. Moreover, the angiogenesis is a transient process with the angiogenic factors may work in an autocrine/paracrine manner.
机译：> 背景/目的： 用来表征小鼠体内 的棕色和白色脂肪组织中冷诱导的血管生成的时空特征，以及体外用？2 3 -肾上腺素受体激动剂（CL316,243）处理的原代小鼠褐色（BA）和白色脂肪细胞（WA）中血管生成因子的时间变化。 方法： 将8周大的雄性C57BL / 6J小鼠分别置于常规笼中，置于冷暴露（4？C）下，分别1、2、3、4和5天。收集肩cap间棕色脂肪组织（iBAT），腹股沟皮下（sWAT）和附睾白色脂肪组织（eWAT）进行免疫组织化学和基因表达分析。 在体外，收集经或不经CL316,243处理的原代小鼠BA和WA进行基因表达和蛋白质分泌分析。 结果： 形态学和遗传学（ Vegfa，Vegfr2，Hif-1？±，Pai1和Pedf ）的组合显示出了仓库特定的血管生成以应对冷暴露。经过CL316,243处理后，BA和WA中的血管生成因子（Vegfa，Vegfr2，Hif-1α±，Pai1和Pedf ）和分泌的蛋白VEGFA均瞬时升高。 结论： 我们的结果表明，iBAT对冷诱导的血管生成具有高度响应，其主要受sWAT支持，而eWAT的作用较小。此外，血管生成是短暂的过程，其中血管生成因子可能以自分泌/旁分泌方式起作用。

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