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首页> 外文期刊>Cellular Physiology and Biochemistry >Peptidomic Analysis of Cultured Cardiomyocytes Exposed to Acute Ischemic-Hypoxia
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Peptidomic Analysis of Cultured Cardiomyocytes Exposed to Acute Ischemic-Hypoxia

机译：暴露于急性缺血性缺氧的心肌细胞的肽分析

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>Background: Acute Myocardial Infarction (AMI) is a life-threatening cardiovascular disease involving disruption of blood flow to the heart, consequent tissue damage, and sometimes death. Peptidomics, an emerging branch of proteomics, has attracted wide attention. Methods: A comparative peptidomic profiling was used to explore changes induced by acute ischemic-hypoxia in primary cultured neonatal rat myocardial cells. Analysis of six-plex tandem mass tag (TMT) labelled peptides was performed using nanoflow liquid chromatography coupled online with an LTQ-Orbitrap Velos mass spectrometer. Results: A total of 220 differentially expressed peptides originating from 119 proteins were identified, of which 37 were upregulated and 183 were downregulated in cardiomyocytes exposed to hypoxia/ischemia conditions. Many of the identified peptides were derived from functional domains of proteins closely associated with cardiomyocyte structure or AMI. Conclusion: Numerous peptides may be involved in process of AMI. These results pave the way for future functional studies of the identified peptides.
机译：> 背景： 急性心肌梗塞（AMI）是一种危及生命的心血管疾病，涉及流向心脏的血流中断，随之而来的组织损伤，有时甚至死亡。肽组学是蛋白质组学的一个新兴分支，已引起广泛关注。 方法： 使用一种比较性的肽谱分析方法，探讨了原代培养的新生大鼠心肌细胞由急性缺血性缺氧引起的变化。使用纳米流液相色谱在线结合LTQ-Orbitrap Velos质谱仪对六重串联质谱标签（TMT）标记的肽进行分析。 结果： 在暴露于缺氧/缺血条件下的心肌细胞中，共鉴定出220种源自119种蛋白质的差异表达肽，其中37种被上调且183个被下调。许多鉴定出的肽衍生自与心肌细胞结构或AMI紧密相关的蛋白质的功能域。 结论： AMI过程可能涉及许多肽。这些结果为所鉴定的肽的未来功能研究铺平了道路。

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