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首页> 外文期刊>Cellular Physiology and Biochemistry >Microarray Expression Profile of Circular RNAs in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients
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Microarray Expression Profile of Circular RNAs in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients

机译：类风湿关节炎患者外周血单个核细胞中环状RNA的芯片表达谱

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>Background/Aims: Circular RNAs (circRNAs) compose a large class of RNAs that can be used as biomarkers in clinical blood samples. This study aimed to determine the expression of circRNAs in peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients to identify novel biomarkers for RA screening. Methods: We started with a microarray screening of circRNA changes in PBMCs from 5 RA patients and 5 healthy controls. We then confirmed the selected circRNA changes in PBMCs from 30 RA patients and 25 age- and sex-matched controls using the real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Spearman correlation test was performed to assess the correlation of circRNAs and clinical variables. Receiver operating characteristic (ROC) curve was calculated to evaluate the diagnostic value. Results: We identified and verified five circRNAs (092516, 003524, 103047, 104871, 101873) that were significantly elevated in PBMCs from RA patients. Among these RA patients, we detected no significant correlation between the five circRNAs and the disease severity, including disease activity score (DAS28), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and health assessment questionnaire (HAQ). Yet, ROC curve analysis suggested that circRNA_104871 has significant value of RA diagnosis (AUC=0.833, P<0.001), followed by circRNA_003524 (AUC = 0.683, P = 0.020), circRNA_101873 (AUC = 0.676, P = 0.026), and circRNA_103047 (AUC = 0.671, P = 0.030). Conclusions: This study suggests that increased expression of circRNAs circRNA_104871, circRNA_003524, circRNA_101873 and circRNA_103047 in PBMC from RA patients may serve as potential biomarkers for RA patient diagnosis.
机译：> 背景/目的： 环状RNA（circRNA）构成了一类可在临床血液样本中用作生物标记物的RNA。这项研究旨在确定circRNA在类风湿关节炎（RA）患者外周血单个核细胞（PBMC）中的表达，以鉴定用于RA筛查的新型生物标志物。 方法： 我们从5例RA患者和5例健康对照的PBMC中circRNA变化的微阵列筛选开始。然后，我们使用实时定量逆转录聚合酶链反应（qRT-PCR）从30位RA患者和25位年龄和性别匹配的对照中确认了PBMC中选定的circRNA变化。进行Spearman相关性测试以评估circRNA与临床变量的相关性。计算接收器工作特性（ROC）曲线以评估诊断值。 结果： 我们鉴定并验证了RA患者的PBMC中显着升高的5个circRNA（092516、003524、103047、104871、101873）。在这些RA患者中，我们未发现这5种circRNA与疾病严重程度之间没有显着相关性，包括疾病活动评分（DAS28），红细胞沉降率（ESR），C反应蛋白（CRP）和健康评估问卷（HAQ）。然而，ROC曲线分析表明circRNA_104871具有RA诊断的显着价值（AUC = 0.833，P <0.001），其次是circRNA_003524（AUC = 0.683，P = 0.020），circRNA_101873（AUC = 0.676，P = 0.026）和circRNA_103047 （AUC = 0.671，P = 0.030）。 结论： 该研究表明，RA患者的PBMC中circRNA circRNA_104871，circRNA_003524，circRNA_101873和circRNA_103047的表达增加可能是诊断RA患者的潜在生物标志物。

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