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首页> 外文期刊>Cellular Physiology and Biochemistry >MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/?2-Catenin Signaling Pathway
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MiR-129-5p Inhibits Proliferation and Invasion of Chondrosarcoma Cells by Regulating SOX4/Wnt/?2-Catenin Signaling Pathway

机译：MiR-129-5p通过调节SOX4 / Wnt /β2-catenin信号通路抑制软骨肉瘤细胞的增殖和侵袭

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>Background/Aims: Recently, microRNAs (miRNA) have been identified as novel regulators in Chondrosarcoma (CHS). This study was aimed to identify the roles of miR-129-5p-5p in regulation of SOX4 and Wnt/?2-catenin signaling pathway, as well as cell proliferation and apoptosis in chondrosarcomas. Materials and Methods: Tissue samples were obtained from chondrosarcoma patients. Immunohistochemistry, real-time quantitative RT-PCR (RT-qPCR) and western blot analysis were performed to detect the expressions of miR-129-5p and SOX4. Luciferase assay was conducted to confirm that miR-129-5p directly targeted SOX4 mRNA. Manipulations of miR-129-5p and SOX4 expression were achieved through cell transfection. Cell proliferation, migration and apoptosis were evaluated by CCK-8 assay, colony forming assay, wound healing assay and flow cytometry in vitro. For in vivo experiment, the tumor xenograft model was established to evaluate the effects of miR-129-5p and SOX4 on chondrosarcomas. Results: The expression of miR-129-5p was significantly down-regulated in chondrosarcoma tissues as well as cells in comparison with normal ones, while SOX4 was over-activated. Further studies suggested that miR-129-5p suppressed cell proliferation, migration and promoted apoptosis by inhibiting SOX4 and Wnt/?2-catenin pathway. Conclusion: MiR-129-5p inhibits the Wnt/?2-catenin signaling pathway by targeting SOX4 and further suppresses cell proliferation, migration and promotes apoptosis in chondrosarcomas.
机译：> 背景/目标： 近来，微小RNA（miRNA）被鉴定为软骨肉瘤（CHS）中的新型调节剂。本研究旨在确定miR-129-5p-5p在调节SOX4和Wnt /β2-catenin信号通路以及软骨肉瘤中细胞增殖和凋亡中的作用。 材料和方法： 组织样品来自软骨肉瘤患者。进行了免疫组织化学，实时定量RT-PCR（RT-qPCR）和蛋白质印迹分析，以检测miR-129-5p和SOX4的表达。进行荧光素酶测定以证实miR-129-5p直接靶向SOX4 mRNA。通过细胞转染可操纵miR-129-5p和SOX4的表达。在体外通过CCK-8测定，集落形成测定，伤口愈合测定和流式细胞术评估细胞的增殖，迁移和凋亡。为了进行体内实验，建立了肿瘤异种移植模型以评估miR-129-5p和SOX4对软骨肉瘤的作用。 结果： 与正常组织相比，软骨肉瘤组织和细胞中miR-129-5p的表达明显下调，而SOX4则被过度激活。进一步的研究表明，miR-129-5p通过抑制SOX4和Wnt /β2-catenin途径抑制细胞增殖，迁移并促进细胞凋亡。 结论： MiR-129-5p通过靶向SOX4抑制Wnt /α2-catenin信号传导途径，并进一步抑制软骨肉瘤中的细胞增殖，迁移并促进细胞凋亡。

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