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首页> 外文期刊>Cellular Physiology and Biochemistry >Selection of Hepatocyte-Like Cells from Mouse Differentiated Embryonic Stem Cells and Application in Therapeutic Liver Repopulation
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Selection of Hepatocyte-Like Cells from Mouse Differentiated Embryonic Stem Cells and Application in Therapeutic Liver Repopulation

机译：从小鼠分化的胚胎干细胞中筛选类似于肝细胞的细胞及其在治疗性肝再生中的应用

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biBackgroud/Aim /i/bBecause of the oncogenic risk, it is important to gain the homogeneous and purified cells from differentiated ESCs before transplantation. Here, we aim to select hepatocyte-like cells from differentiated ESCs, and investigate their growth, differentiation and neoplastic formation after intrahepatic transplantation. biMethods /i/bMouse ESCs were primarily induced by Dexamethesone, FGF-4 and HGF sequentially, then placed to a conditioning selection media consisting of 5% cholestatic sera and cultivated for 2 wks. After labeled by CFDA-SE, the selected cells were transplanted into mouse liver in therapeutic liver repopulation models. biResults /i/bIn the early stage of screening cultivation, most cells were suffered from apoptosis or even death. 1w later, some hepatocyte-like colony-forming units were observed, then the selected cells could grow and tend to be more mature, as assessed by morphological and functional tests. After intrahepatic transplantation, the labeled cells could proliferate and expressed albumin. Moreover, teratoma didn’t form over 3 months. biConclusion /i/bOur conditioning selection media could not only effectively select hepatocyte-like cells from differentiated ESCs, but further promote their growth and differentiation as well. After intrahepatic transplantation in therapeutic liver repopulation models, the selected cells could grow, differentiate and keep partial hepatic function. In particular, the transplantation was safe.
机译：Backgroud / Aim 由于致癌风险，重要的是在移植前从分化的ESC中获得均质和纯化的细胞。在这里，我们旨在从分化的胚胎干细胞中选择肝细胞样细胞，并研究其在肝内移植后的生长，分化和肿瘤形成。 方法 首先由地塞米松，FGF-4和HGF诱导小鼠ESC，然后将其置于包含5％胆汁淤积血清的条件选择培养基中并培养2周。在通过CFDA-SE标记后，将选定的细胞以治疗性肝再造模型移植到小鼠肝中。 结果 在筛选培养的早期，大多数细胞都发生凋亡甚至死亡。 1w后，通过形态学和功能测试评估，观察到一些肝细胞样集落形成单位，然后所选择的细胞可以生长并趋于成熟。肝内移植后，标记的细胞可以增殖并表达白蛋白。而且，畸胎瘤没有形成超过三个月。 结论 我们的条件选择培养基不仅可以有效地从分化的ESC中选择肝细胞样细胞，而且还可以促进它们的生长和分化。在治疗性肝移植模型中进行肝内移植后，所选细胞可以生长，分化并保持部分肝功能。尤其是，移植是安全的。

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