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首页> 外文期刊>Cellular Physiology and Biochemistry >Clinical Significance of miR-210 and its Prospective Signaling Pathways in Non-Small Cell Lung Cancer: Evidence from Gene Expression Omnibus and the Cancer Genome Atlas Data Mining with 2763 Samples and Validation via Real-Time Quantitative PCR
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Clinical Significance of miR-210 and its Prospective Signaling Pathways in Non-Small Cell Lung Cancer: Evidence from Gene Expression Omnibus and the Cancer Genome Atlas Data Mining with 2763 Samples and Validation via Real-Time Quantitative PCR

机译：miR-210及其潜在信号通路在非小细胞肺癌中的临床意义：来自基因表达综合和2763个样本的癌症基因组图谱数据挖掘的证据，并通过实时定量PCR进行验证

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Background/Aims Since the function of microRNA (miR)-210 in non-small cell lung cancer (NSCLC) remains unclear, we aimed to explore the clinical significance of miR-210 in NSCLC. Methods NSCLC-related data from 1673 samples on Gene Expression Omnibus and 1090 samples on The Cancer Genome Atlas were obtained and analyzed. The expression level of miR-210 was validated via real-time quantitative PCR analysis with 125 paired clinical samples. A meta-analysis was performed to generate a comprehensive understanding of miR-210 expression and its clinical significance in NSCLC. In addition, bioinformatics analysis was also conducted to reveal the potential underlying mechanism of miR-210 action in NSCLC. Results miR-210 expression was consistently elevated in NSCLC solid tissue samples. However, its expression was controversial in easily obtained body fluids (i.e., blood, plasma, and serum). Moreover, an overall pooled meta-analysis implied a comparatively higher level of miR-210 expression in NSCLC cancerous tissue than in normal control tissue (P < 0.001). In addition, a meta-analysis of outcome revealed a significant diagnostic capacity of miR-210 in NSCLC by detecting its expression in serum and sputum (area under the summary receiver operating characteristic curve 0.82 and 0.81, respectively). miR-210 overexpression was associated with poor progression-free survival (PFS) in NSCLC and was negatively related to overall survival and disease-free survival. Bioinformatic gene enrichment and annotation analyses showed that the target genes of miR-210 were greatly enriched in cell adhesion and plasma membrane, and three pathways were considered to be the main functional circuits of miR-210 renin secretion, the cGMP-PKG signaling pathway, and cell adhesion molecules. Conclusion In NSCLC, miR-210 expression was elevated and overexpression indicated poor PFS. Expression level of miR-210 in serum and sputum showed significant diagnostic value for NSCLC.
机译：背景/目的由于microRNA（miR）-210在非小细胞肺癌（NSCLC）中的功能尚不清楚，因此我们旨在探讨miR-210在NSCLC中的临床意义。方法从基因表达综合中的1673个样本和癌症基因组图谱中的1090个样本中获得与NSCLC相关的数据并进行分析。 miR-210的表达水平通过实时定量PCR分析与125个配对的临床样品进行验证。进行荟萃分析以全面了解miR-210表达及其在NSCLC中的临床意义。此外，还进行了生物信息学分析以揭示miR-210在非小细胞肺癌中的潜在潜在机制。结果在NSCLC实体组织样品中，miR-210的表达持续升高。然而，其表达在容易获得的体液（即血液，血浆和血清）中存在争议。此外，整体汇总荟萃分析表明，NSCLC癌组织中的miR-210表达水平高于正常对照组织中的水平（P＆＃x3c; 0.001）。此外，结果的荟萃分析通过检测miR-210在血清和痰中的表达（在总受体工作特征曲线下的区域，分别为0.82和0.81）显示出在NSCLC中具有显着的诊断能力。 miR-210的过表达与NSCLC的不良无进展生存期（PFS）相关，并且与总体生存期和无病生存期呈负相关。生物信息学基因富集和注释分析表明，miR-210的靶基因在细胞黏附和质膜上大量富集，并且三个途径被认为是miR-210肾素分泌的主要功能电路，即cGMP-PKG信号传导途径，和细胞粘附分子。结论在NSCLC中，miR-210表达升高，过表达表明PFS较差。血清和痰中miR-210的表达水平对NSCLC具有重要的诊断价值。

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