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首页> 外文期刊>Cellular Physiology and Biochemistry >Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques
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Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

机译：源自ADM支架的转基因小鼠骨髓间充质干细胞在伤口愈合中的活性并通过先进的光学技术进行评估

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>Background/Aims: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. Methods: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing. Results: GFP-tagged MSCs were retained inside the regenerating skin until day 14 post-transplantation. Alpha-smooth muscle actin (?±-SMA) and vimentin (VIM) were detected at 3, 5, 7, and 14 days post-transplantation by immunofluorescence double labeling. Although the GFP+/?±-SMA+- and GFP+/VIM+-cell numbers decreased gradually with healing time, ?±-SMA+- and VIM+-cell numbers significantly increased, most of them were endogenous functional cells which were related to angiogenesis and collagen fiber structural remodeling. Conclusion: Therefore, in the initial stage of wound healing, transplanted MSCs differentiated into functional cells and played paracrine roles to recruit more endogenous cells for tissue remodeling. With the disappearance of exogenous cells, endogenous cells were responsible for the latter stage of cutaneous wound healing.
机译：> 背景/目标 ：骨髓间充质干细胞（MSC）有望通过增强细胞过程促进皮肤伤口愈合。我们评估了GFP标记的MSCs移植对小鼠切除伤口皮肤再生的影响。 方法 ：将GFP标记的转基因小鼠的MSC与脱细胞真皮基质（ADM）支架共培养，并将MSC-ADM支架移植到BALB / c只小鼠。植入后，通过二次谐波和双光子激发荧光成像，蛋白质印迹以及DNA扩增和测序检查MSC的存活和行为。 结果 ：GFP标记的MSCs保留在再生皮肤内部，直到移植后第14天。通过免疫荧光双标记法在移植后第3、5、7和14天检测到α平滑肌肌动蛋白（α±SMA）和波形蛋白（VIM）。尽管GFP + /？±-SMA + -和GFP + / VIM + -细胞数逐渐减少随着愈合时间的延长，α±SMA + -和VIM + -细胞数量显着增加，其中大多数为内源性功能细胞，与血管新生和胶原纤维结构重塑有关。 。 结论 ：因此，在伤口愈合的初始阶段，移植的MSC分化为功能性细胞并发挥旁分泌作用，以募集更多内源性细胞进行组织重塑。随着外源细胞的消失，内源性细胞负责皮肤伤口愈合的后期。

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