...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >Influence of Dexamethasone on Na+/H+ Exchanger Activity in Dendritic Cells
【24h】

Influence of Dexamethasone on Na+/H+ Exchanger Activity in Dendritic Cells

机译：地塞米松对树突状细胞Na + / H +交换子活性的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glucocorticoids regulate the function of dendritic cells (DCs), antigen-presenting cells linking innate and adaptive immunity. Glucocorticoids influence the function of other cell types by modulating the activity of the Nasup+/sup/Hsup+/supexchanger (NHE), a carrier involved in the regulation of cytosolic pH and cell volume. The present study explored whether dexamethasone influences Nasup+/sup/Hsup+/sup exchanger activity in DCs. The DCs were isolated from mouse bone marrow, cell volume was estimated from forward scatter in FACS analysis, cytosolic pH (pHsubi/sub) utilizing BCECF fluorescence and Nasup+/sup/Hsup+/sup exchanger activity from the Nasup+/sup dependent realkalinization after an ammonium pulse. Treatment with the glucocorticoid dexamethasone (100 nM; 1, 4, 16 and 24h) significantly decreased pHsubi/sub (≧4 h) and gradually increased Nasup+/sup/Hsup+/sup exchanger activity (=16 h). The stimulation of Nasup+/sup/Hsup+/sup exchanger activity by dexamethasone was virtually abrogated by glucocorticoid receptor blocker mefiprestone (1 µM) and NHE3 inhibitor dimethyl amiloride (5 µM), but not prevented by NHE1 inhibitor cariporide (10 µM). Dexamethasone treatment significantly increased SGK1 mRNA levels. Stimulation of Nasup+/sup/Hsup+/sup exchanger activity by dexamethasone was blunted in DCs lacking SGK1. Dexamethasone treatment did not significantly alter ROS formation but significantly decreased the forward scatter. Exposure of DCs to lipopolysacharide (LPS, 1 µg/ml) led to a transient increase followed by a decline of Nasup+/sup/Hsup+/sup exchanger activity and to enhanced forward scatter as well as ROS formation, all effects significantly blunted in the presence of dexamethasone (100 nM). In conclusion, glucocorticoid treatment decreased pHsubi/sub and cell volume, effects paralleled by upregulation of Nasup+/sup/Hsup+/sup exchanger activity in DCs. Moreover, glucocorticoids blunted the stimulation of Nasup+/sup/Hsup+/sup exchanger activity, cell swelling and ROS formation following LPS treatment.
机译：糖皮质激素调节树突状细胞（DC）的功能，树突状细胞是连接先天免疫和适应性免疫的抗原呈递细胞。糖皮质激素可通过调节Na + / H + 交换剂（NHE）的活性来影响其他细胞类型的功能，NHE是一种参与调节细胞质pH和细胞体积的载体。本研究探讨了地塞米松是否影响DC中Na + / H + 交换子的活性。从小鼠骨髓中分离DC，通过FACS分析的前向散射，使用BCECF荧光和Na + / H i ）估计细胞体积铵脉冲后Na + 依赖的碱化作用产生的> + 交换子活性。用糖皮质激素地塞米松（100 nM; 1，4，4，16和24h）处理可显着降低pH i （≧4 h），并逐渐增加Na + / H + 交换器活动（= 16小时）。地塞米松对Na + / H + 交换子的刺激作用实际上已被糖皮质激素受体阻滞剂甲氧普列酮（1 µM）和NHE3抑制剂二甲基阿米洛利（5 µM）废除，但没有由NHE1抑制剂cariporide（10 µM）预防。地塞米松治疗显着增加了SGK1 mRNA水平。在缺乏SGK1的DC中，地塞米松对Na + / H + 交换子活性的刺激作用减弱。地塞米松治疗并未显着改变ROS的形成，但显着降低了前向散射。 DCs暴露于脂多糖（LPS，1 µg / ml）导致瞬时增加，随后Na + / H + 交换子活性下降，并导致前向散射增强。以及ROS的形成，在地塞米松（100 nM）存在下所有作用均明显减弱。总之，糖皮质激素治疗可降低DC中pH i 和细胞体积，其作用与Na + / H + 交换子活性的上调平行。此外，糖皮质激素抑制了LPS处理后Na + / H + 交换子活性，细胞肿胀和ROS形成的刺激。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号