...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >Extracellular GTP is a Potent Water-Transport Regulator via Aquaporin 5 Plasma-Membrane Insertion in M1-CCD Epithelial Cortical Collecting Duct Cells
【24h】

Extracellular GTP is a Potent Water-Transport Regulator via Aquaporin 5 Plasma-Membrane Insertion in M1-CCD Epithelial Cortical Collecting Duct Cells

机译：细胞外GTP是有效的水运输调节剂，通过在M1-CCD上皮皮质收集管细胞中的Aquaporin 5质膜插入而实现。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

biBackground/Aims /i/bExtracellular GTP is able to modulate some specific functions in neuron, glia and muscle cell models as it has been demonstrated over the last two decades. In fact, extracellular GTP binds its specific plasma membrane binding sites and induces signal transduction via [Casup2+/sup]subi/sub increase. We demonstrate, for the first time, that extracellular GTP is able to modulate cell swelling in M1-CCD cortical collecting duct epithelial cells via upregulation of aquaporin 5 (AQP5) expression. biMethods /i/bWe used videoimaging, immunocitochemistry, flow cytometry, confocal techniques, Western blotting and RT-PCR for protein and gene expression analysis, respectively. biResults /i/bWe demonstrate that AQP5 mRNA is up-regulated 7 h after the GTP exposure in the cell culture medium, and its protein level is increased after 12-24 h. We show that AQP5 is targeted to the plasma membrane of M1-CCD cells, where it facilitates cell swelling, and that the GTP-dependent AQP5 up-regulation occurs via [Casup2+/sup]subi/sub increase. Indeed, GTP induces both oscillating and transient [Casup2+/sup]subi/sub increase, and specifically the oscillating kinetic appears to be responsible for blocking cell cycle in the S-phase while the [Casup2+/sup]subi/sub influx, with whatever kinetic, seems to be responsible for inducing AQP5 expression. biConclusion /i/bThe role of GTP as a regulator of AQP5-mediated water transport in renal cells is of great importance in the physiology of renal epithelia, due to its possible physiopathological implications. GTP-dependent AQP5 expression could act as osmosensor. In addition, the data presented here suggest that GTP might play the same role in other tissues where rapid water transport is required for cell volume regulation and maintenance of the homeostasis.
机译：背景/目标 细胞外GTP能够调节神经元，神经胶质和肌肉细胞模型中的某些特定功能，这在过去的二十年中已得到证实。实际上，细胞外GTP结合其特定的质膜结合位点，并通过[Ca 2 + ] i 的增加诱导信号转导。我们首次证明，细胞外GTP能够通过上调水通道蛋白5（AQP5）的表达来调节M1-CCD皮质收集导管上皮细胞中的细胞肿胀。 方法 我们分别使用视频成像，免疫细胞化学，流式细胞术，共聚焦技术，蛋白质印迹和RT-PCR进行蛋白质和基因表达分析。 结果 我们证明AQP5 mRNA在细胞培养基中GTP暴露后7小时被上调，并且其蛋白质水平在12-24小时后升高。我们表明，AQP5靶向M1-CCD细胞的质膜，它促进细胞肿胀，并且依赖于GTP的AQP5上调通过[Ca 2 + ] i发生增加。的确，GTP诱导了振荡和瞬时[Ca 2 + ] i 的增加，特别是振荡动力学似乎是阻止S期细胞周期的原因。 [Ca 2 + ] i 的涌入，无论其动力学如何，似乎都可以诱导AQP5表达。 结论 由于其可能的生理病理学意义，GTP作为AQP5介导的肾细胞水运输调节剂的作用在肾上皮的生理中非常重要。 GTP依赖的AQP5表达可作为渗透传感器。此外，此处提供的数据表明，GTP可能在需要快速输水以调节细胞体积和维持体内平衡的其他组织中发挥相同的作用。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号