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首页> 外文期刊>Cellular Physiology and Biochemistry >Erythropoietin-Derived Peptide Protects Against Acute Lung Injury After Rat Traumatic Brain Injury
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Erythropoietin-Derived Peptide Protects Against Acute Lung Injury After Rat Traumatic Brain Injury

机译：促红细胞生成素衍生的肽可预防大鼠脑外伤后的急性肺损伤

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>Background: Traumatic brain injury (TBI) can be complicated by TBI-triggered acute lung injury (ALI), in which inflammation plays a central role. It has been reported that an Erythropoietin-derived peptide (pHBSP) was able to ameliorate TBI; however, its function in TBI-caused ALI has not been reported yet. Methods: In this study, we studied the effect of pHBSP on TBI-caused ALI by using a weight-drop induced TBI model. At 8 h and 24 h post-TBI, pulmonary edema (PE) and bronchoalveolar lavage fluid (BALF) proteins were measured, and haematoxylin and eosin (H&E) staining of lung sections was carried out. At 24 h following TBI, the lungs were harvested for immunofluorescence staining and qRT-PCR analysis. Results: At 8 h and 24 h post-TBI, pHBSP treatment significantly decreased wet/dry ratios, decreased total BALF protein, and attenuated the histological signs of pulmonary injury. At 24 h post-TBI, pHBSP treatment decreased the accumulation of CD68+ macrophages in the lung and reduced the mRNA levels of TNF-?±, IL-6, IL-1?2 and iNOS in the lung. Conclusions: We identified the protective role that pHBSP played in TBI-caused ALI, suggesting that pHBSP is a potent candidate for systemic therapy in TBI patients.
机译：> 背景： 外伤性脑损伤（TBI）可以由TBI触发的急性肺损伤（ALI）并发，其中炎症起主要作用。据报道，促红细胞生成素衍生的肽（pHBSP）能够改善TBI。但是，尚未报道它在由TBI引起的ALI中的功能。 方法： 在这项研究中，我们使用重量减轻的TBI模型研究了pHBSP对TBI引起的ALI的影响。在TBI后8小时和24小时，测量肺水肿（PE）和支气管肺泡灌洗液（BALF）蛋白，并对肺切片进行苏木精和曙红（H＆E）染色。 TBI后24小时，收集肺用于免疫荧光染色和qRT-PCR分析。 结果： TBI后8小时和24小时，pHBSP处理显着降低了干/湿比，降低了BALF的总蛋白，并减轻了肺损伤的组织学征象。 TBI后24小时，pHBSP处理可减少肺中CD68 + 巨噬细胞的积累，并降低TNF-α，IL-6，IL-1β2和iNOS的mRNA水平。肺。 结论： 我们确定了pHBSP在TBI引起的ALI中的保护作用，这表明pHBSP是TBI患者全身治疗的有效候选者。

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