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首页> 外文期刊>Cellular Physiology and Biochemistry >H19 Functions as a Competing Endogenous RNA to Regulate EMT by Sponging miR-130a-3p in Glioma
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H19 Functions as a Competing Endogenous RNA to Regulate EMT by Sponging miR-130a-3p in Glioma

机译：H19通过在胶质瘤中海绵化miR-130a-3p作为竞争性内源RNA调节EMT

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Background/Aims Glioma is one of the most devasting tumors and confers dismal prognosis. Long noncoding RNAs(lncRNAs) have emerged as important regulators in various tumors including glioma. A classic lncRNA-H19, which is found to be highly expressed in human glioma tissues and cell lines, and is associated with tumor progression thus predicating clinical outcomes in glioma patients. However, the overall biological functions and their mechanism of H19 in glioma are not fully understood. Methods Firstly, we analyzed H19 alterations in different grades of glioma tissues through an analysis of 5 sequencing datasets and qRT-PCR was performed to confirm the results. Next, we evaluated the effect of H19 on glioma cells migration, invasion and EMT process. Luciferase assays and RIP assays were employed to figure out the correlation of H19 and SOX4. Results H19 was overexpressed in glioma tissues. Down-regulation of H19 led to the inhibition of migration, invasion and EMT process with a reduction in N-cadherin and Vimentin. H19 and SOX4 are both direct target of miR-130a-3p. H19 could compete with SOX4 via sponging miR-130a-3p. Conclusion Taken together, these results provide a possible function of H19 as an oncogene in glioma tissues and provide a potential new therapeutic strategy for human glioma.
机译：背景/目的脑胶质瘤是最具破坏性的肿瘤之一，预后不良。长的非编码RNA（lncRNAs）已成为包括胶质瘤在内的多种肿瘤的重要调控因子。发现经典的lncRNA-H19在人类神经胶质瘤组织和细胞系中高表达，并且与肿瘤进展相关，因此可预测神经胶质瘤患者的临床结局。但是，还没有完全了解神经胶质瘤中H19的整体生物学功能及其机制。方法：首先，我们通过分析5个测序数据集来分析不同等级的神经胶质瘤组织中H19的变化，并进行qRT-PCR以确认结果。接下来，我们评估了H19对神经胶质瘤细胞迁移，侵袭和EMT过程的影响。使用萤光素酶测定法和RIP测定法来确定H19和SOX4的相关性。结果H19在神经胶质瘤组织中过表达。 H19的下调导致N-钙黏着蛋白和波形蛋白的减少，从而抑制迁移，侵袭和EMT过程。 H19和SOX4都是miR-130a-3p的直接靶标。 H19可以通过海绵miR-130a-3p与SOX4竞争。结论综上所述，这些结果提供了H19在神经胶质瘤组织中作为癌基因的可能功能，并为人神经胶质瘤提供了潜在的新治疗策略。

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