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首页> 外文期刊>Cellular Physiology and Biochemistry >Alterations in Pancreatic Protein Expression in STZ-Induced Diabetic Rats and Genetically Diabetic Mice in Response to Treatment with Hypoglycemic Dipeptide Cyclo (His-Pro)
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Alterations in Pancreatic Protein Expression in STZ-Induced Diabetic Rats and Genetically Diabetic Mice in Response to Treatment with Hypoglycemic Dipeptide Cyclo (His-Pro)

机译：降糖二肽Cyclo（His-Pro）治疗对STZ诱导的糖尿病大鼠和遗传性糖尿病小鼠胰腺蛋白表达的影响

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To provide insights into the molecular mechanisms underlying diabetes mellitus, we performed a proteomic study on two diabetic animal models, streptozotocin (STZ)-induced diabetic rats (T1DM) and genetically diabetic (C57BL/6J iob/ob/i) mice (T2DM). To better understand the recovery process of those diabetic rodents, we examined the effect of hypoglycemic dipeptide Cyclo (His-Pro) (CHP) treatment on the differential expression of pancreatic proteins in both animal models. Oral administration of CHP had an excellent hypoglycemic effect in both animal models, lowering the average plasma glucose level by over 50%. Pancreatic proteins were separated by two-dimensional gel electrophoresis (2-DE) and identified by MALDI-TOF mass spectrometry. This study allowed, for the first time, the identification of 34 proteins that are related to diabetes and potential targets of CHP, a potent anti-diabetic agent for both T1DM and T2DM. The alterations in the expression of these proteins could indicate a tendency for diabetic animals to overcome their diabetic state. These proteins are involved in cellular functions such as metabolism, cellular structure, oxidative stress, as well as signal and energy transduction. Some have already been linked to diabetes, suggesting that the newly identified proteins might also be significant in the etiology of this pathology and should be further investigated. Furthermore, CHP has emerged as a potent tool for both the treatment and study of the molecular mechanisms underlying diabetes. Thus, the findings presented here provide new insights into the study and potential treatment of this pathology.
机译：为了深入了解糖尿病的分子机制，我们对两种糖尿病动物模型进行了蛋白质组学研究，即链脲佐菌素（STZ）诱导的糖尿病大鼠（T1DM）和遗传性糖尿病（C57BL / 6J ob / ob ）小鼠（T2DM）。为了更好地了解这些糖尿病啮齿动物的恢复过程，我们在两个动物模型中检查了降血糖二肽环（His-Pro）（CHP）处理对胰腺蛋白差异表达的影响。在两种动物模型中，口服CHP均具有出色的降血糖作用，可使平均血浆葡萄糖水平降低50％以上。胰蛋白通过二维凝胶电泳（2-DE）分离，并通过MALDI-TOF质谱进行鉴定。这项研究首次鉴定了与糖尿病和CHP潜在靶标有关的34种蛋白质，CHP是T1DM和T2DM的有效抗糖尿病药。这些蛋白质表达的改变可能表明糖尿病动物有克服其糖尿病状态的趋势。这些蛋白质参与细胞功能，例如新陈代谢，细胞结构，氧化应激以及信号和能量转导。一些已经与糖尿病有关，这表明新发现的蛋白质在这种病理的病因中可能也很重要，应该进一步研究。此外，CHP已成为治疗和研究糖尿病潜在分子机制的有效工具。因此，这里介绍的发现为这种病理学的研究和潜在治疗提供了新的见解。

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