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首页> 外文期刊>Central European Journal of Biology >A Preliminary Study on the Newly Isolated High Laccase-producing Fungi: Screening, Strain Characteristics and Induction of Laccase Production
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A Preliminary Study on the Newly Isolated High Laccase-producing Fungi: Screening, Strain Characteristics and Induction of Laccase Production

机译：新分离的高产产漆酶的真菌的初步研究：产漆酶的筛选，菌株特性和诱导

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A simple separation method was used in this study to directly separate laccase-producing fungi from withered plant materials. A laccase-producing filamentous fungus was isolated and purified. The strain was highly similar to the species in genus emTrametes/em by ITS sequence analysis, and therefore named emTrametes/em sp. MA-X01. The addition of cupric ions and aromatic compounds to the liquid medium could induce the laccase synthesis in emTrametes/em sp. MA-X01. Copper-induced laccase activity increased in a dose-dependent manner. The highest laccase activity (2138.9 ± 340.2 U/L) was obtained by adding 2.5 mM Cusup2+/sup to the culture medium, which was about 7 times higher than that of the control group. The induction degree of aromatic compounds was different. For the present study, the highest laccase activities were obtained by adding vanillic acid (1007.9±59.5 U/L) or vanillin (981.6±77.2 U/L) to the medium, which were 3.5 and 3.4 times higher than the laccase activity of the control group, respectively.
机译：在这项研究中使用一种简单的分离方法直接从枯萎的植物材料中分离出产生漆酶的真菌。分离并纯化产生漆酶的丝状真菌。通过ITS序列分析，该菌株与 Trametes 属的物种高度相似，因此被命名为 Trametes sp。 MA-X01。在液体培养基中添加铜离子和芳香族化合物可以诱导 Trametes sp。中漆酶的合成。 MA-X01。铜诱导的漆酶活性以剂量依赖性方式增加。通过向培养基中添加2.5 mM Cu 2 + ，获得最高的漆酶活性（2138.9±340.2 U / L），约为对照组的7倍。芳香族化合物的诱导程度不同。在本研究中，通过向培养基中添加香草酸（1007.9±59.5 U / L）或香兰素（981.6±77.2 U / L）获得最高的漆酶活性，分别是其漆酶活性的3.5和3.4倍。对照组。

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