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Characterization of humic acids derived from Leonardite using a solid-state NMR spectroscopy and effects of humic acids on growth and nutrient uptake of snap bean

机译:固态NMR光谱表征伦氏沸石的腐殖酸及其对菜豆生长和养分吸收的影响。

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Understanding chemistry and the effects of humic substances on plant growth is important for using organic fertilizer or soil amendment for sustainable snap bean production. The objective of this study was to characterize different fractions of humic acid (HA) derived from Leonardite and evaluate their effects on seedling growth and nutrient uptake of snap bean (Phaseolus vulgaris L.). HAs extracted from Leonardite were separated based on molecular weights into three fractions (HS1, HS2, HS3) plus sediment (SED). With direct polarization combined with spectral editing techniques, functional groups of HAs were quantified and the results indicated that HAs with low molecular weights had more O-alkyl and carboxyl C groups than those with large molecular sizes. A plant growth experiment was conducted as a randomized split-plot design with three replications and repeated for three plantings. The results show that addition of HAs was beneficial to leaf and root growth of snap bean compared with the control (no HA). Plants treated with low-molecular weight HAs had significantly greater root length, root surface area, and uptake of potassium by shoot than those treated with other HAs, while leaf growth was affected mainly by HAs with high molecular weight.
机译:了解化学和腐殖质对植物生长的影响,对于使用有机肥料或土壤改良剂可持续生产四季豆至关重要。这项研究的目的是表征源自伦纳石的腐殖酸(HA)的不同馏分,并评估其对菜豆(Phaseolus vulgaris L.)幼苗生长和养分吸收的影响。从伦纳石中提取的HAs根据分子量分为三个部分(HS1,HS2,HS3)和沉淀物(SED)。通过直接极化结合光谱编辑技术,对HA的官能团进行了定量,结果表明,低分子量的HA具有比大分子的HA更多的O-烷基和羧基C基团。进行植物生长实验,作为随机分裂样地设计,重复三遍,并重复三遍种植。结果表明,与对照(无HA)相比,添加HA有利于四季豆的叶和根生长。用低分子量HA处理的植物的根长,根表面积和枝条对钾的吸收均明显高于其他HA处理的植物,而叶片生长主要受高分子量HA的影响。

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