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Preparative Fractionation Analysis of Acaciapolyacantha Gum Using Acetone as a Solvent

机译:以丙酮为溶剂的阿拉伯胶的制备级分分析

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Five authentic Acacia polyacantha whole gum samples were collected as natural exudates and five acetone fractions were obtained. Analysis of the whole gum samples and the fractions were carried out t for nitrogen (protein), specific rotation, intrinsic viscosity, pH, equivalent weight, uronic acid content, refractive index and average molecular weight. Results of refractive index for the whole samples and the fractions were found to share the same value (1.3337). Significant differences were obtained between the values of these parameters for the whole gum samples as well as the fractions and it is also among the fractions themselves.In particular fractions 1,2,3,4and 5 showed nitrogen content of 0.32%, 0.35%, 0.40%, 0.39%and0.37% and protein content of (2.11%,2.31%,2.64%, 2.57% and2.44%) respectively. The mean values of specific optical rotation of the fractions was found-13.5 0, -14.50, -15.0 0, -16.00 and -14.50 respectively. The average molecular weight of the fractions gave 2.669x105, 2.669X105, 2.714X105, 2.339X105 and 2.754X105 respectively. The statistical analysis showed significant differences (P ≤ 0.05) for some parameters such as, intrinsic viscosity, pH and the average molecular weight. These data confirms the heterogeneity of Acacia polyacantha gum, and their fractions.
机译:收集了五个纯天然阿拉伯胶全树胶样品作为天然分泌物,并获得了五个丙酮馏分。对整个口香糖样品和级分进行氮,蛋白质,比旋光度,特性粘度,pH,当量,糖醛酸含量,折射率和平均分子量的分析。发现整个样品和各部分的折射率结果具有相同的值(1.3337)。整个口香糖样品以及各组分之间的参数值之间都存在显着差异,尤其是组分1,2,3,4和5的氮含量分别为0.32%,0.35%,1,2,3,4和5。蛋白质含量分别为0.40%,0.39%和0.37%(2.11%,2.31%,2.64%,2.57%和2.44%)。各级分的比旋光度的平均值分别为-13.5 0,-14.50,-15.0 0,-16.00和-14.50。馏分的平均分子量分别为2.669×105、2.669×105、2.714×105、2.339×105和2.754×105。统计分析显示某些参数(例如特性粘度,pH和平均分子量)存在显着差异(P≤0.05)。这些数据证实了相思树胶及其各部分的异质性。

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