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首页> 外文期刊>Chilean Journal of Agricultural Research >Hypoxia treatment on germinating faba bean ( Vicia faba L.) seeds enhances GABA-related protection against salt stress
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Hypoxia treatment on germinating faba bean ( Vicia faba L.) seeds enhances GABA-related protection against salt stress

机译:对发芽的蚕豆(蚕豆)种子进行缺氧处理可增强GABA相关的抗盐胁迫保护作用

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The γ-aminobutyric acid (GABA) is a non-protein amino acid with some functional properties for human health. Its content is usually lower in plant seeds. Hypoxia or salt (NaCl) stress is an effective way for accumulating GABA during seed germination. However, NaCl stress on GABA accumulation under hypoxia is currently infrequent. The effect of NaCl on GABA accumulation in germinating faba bean ( Vicia faba L.) under hypoxia was therefore investigated in this study. Faba bean seeds were steeped in citric acid buffer (pH 3.5) containing NaCl with a final O2 concentration of 5.5 mg L-1 and germinated for 5 d. Results showed that 60 mmol L-1 NaCl was the optimum concentration for GABA accumulation in germinating faba beans under hypoxia. Germination for 5 d under hypoxia-NaCl stress was less beneficial for GABA accumulation than only hypoxia (control). Polyamine degradation pathway played a more important role for accumulating GABA in germinating faba bean as an adaptive response to NaCl stress. Removing NaCl significantly increased GABA content, while it decreased glutamate decarboxylase (GAD) activity. Simultaneously, polyamine was accumulated, which might be related to the enhancement of physiological activity after recovery. When treated with aminoguanidine (AG) for 3 d, GABA content decreased by 29.82%. These results indicated that the tolerance ability of GABA shunt to NaCl stress was weaker than that of polyamine degradation pathway. The NaCl treatment for 3 d under hypoxia could raise the contribution ratio of polyamine degradation pathway for GABA accumulation. The contribution ratio of polyamine degradation pathway for GABA formation was 29.82% when treated for at least 3 d.
机译:γ-氨基丁酸(GABA)是一种非蛋白质氨基酸,对人体健康具有某些功能。它的含量通常在植物种子中较低。低氧或盐(NaCl)胁迫是在种子发芽过程中累积GABA的有效方法。然而,NaCl在缺氧条件下对GABA积累的胁迫目前很少见。因此,本研究研究了NaCl对缺氧条件下发芽蚕豆(Vicia faba L.)GABA积累的影响。将蚕豆种子浸入含有NaCl的柠檬酸缓冲液(pH 3.5)中,O 2 的最终浓度为5.5 mg L -1 ,并萌发5 d。结果表明,缺氧条件下60 mmol L -1 NaCl是发芽蚕豆中GABA积累的最佳浓度。在缺氧-NaCl胁迫下发芽5天对GABA积累的好处不如仅缺氧(对照)。多胺降解途径在发芽蚕豆中积累GABA作为对NaCl胁迫的适应性反应起着更重要的作用。除去NaCl会显着增加GABA含量,同时会降低谷氨酸脱羧酶(GAD)活性。同时,多胺积累,可能与恢复后生理活性的增强有关。用氨基胍(AG)处理3 d后,GABA含量降低了29.82%。这些结果表明,GABA分流器对NaCl胁迫的耐受能力弱于多胺降解途径。缺氧条件下NaCl处理3 d可以提高多胺降解途径对GABA积累的贡献率。处理至少3 d后,多胺降解途径对GABA形成的贡献率为29.82%。

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