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首页> 外文期刊>CHROMATOGRAPHY >Selective Extraction of Nucleotides with Fluorous Biphasic System Utilizing Perfluoroalkylamine as an Ion-Pair Reagent
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Selective Extraction of Nucleotides with Fluorous Biphasic System Utilizing Perfluoroalkylamine as an Ion-Pair Reagent

机译：以全氟烷基胺为离子对试剂的荧光双相体系选择性提取核苷酸

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A novel and selective extraction method for nucleotides (AMP, ADP, ATP, GMP, GDP, and GTP) has been developed. In this study, we employed liquid-liquid based fluorous biphasic extraction system in the presence of a 4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-heptadecafluoroundecylamine (HFUA) as an ion-pair reagent. The ion-pairs between nucleotides and HFUA were selectively extracted to a mixture of tetradecafluorohexane and 1H ,1H ,2H ,2H -tridecafluoro-1-n -octanol (3:1, v/v) as fluorous solvent via the affinity of perfluoroalkyl-containing compounds with each other, i.e. fluorous interaction. Then, the nucleotides in the fluorous solvent could be back-extracted with aqueous ammonia in acetonitrile as non-fluorous solvent, and the extracts were directly injected to LC system. A hydrophilic interaction chromatography was utilized for separation of the nucleotides by gradient elution with the mixture of acetonitrile,water, and ammonium carbonate buffer (pH 10.0) as mobile phase, and an ultraviolet detection at 254 nm was used. The extraction recoveries of nucleotides were in the range from 70.4 to 96.8%. This method could be applied to the determination of nucleotides in cell samples.
机译：已经开发了一种新颖的，选择性的核苷酸提取方法（AMP，ADP，ATP，GMP，GDP和GTP）。在这项研究中，我们在存在4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11的情况下采用液-液基双相萃取系统，11-十七氟十一烷基胺（HFUA）作为离子对试剂。选择性地将核苷酸和HFUA之间的离子对提取到十四氟己烷和1 H，1 H，2 H，2 H -tridecafluoro-1- n-的混合物中辛醇（3：1，v / v）作为含氟溶剂，通过全氟烷基化合物相互之间的亲和力，即氟相互作用。然后，可以用乙腈中的氨水作为非氟溶剂反萃取氟溶剂中的核苷酸，并将提取物直接注入液相色谱系统中。利用亲水相互作用色谱法，通过以乙腈，水和碳酸铵缓冲液（pH 10.0）的混合物作为流动相，通过梯度洗脱来分离核苷酸，并使用254 nm的紫外检测。核苷酸的提取回收率在70.4至96.8％的范围内。该方法可用于细胞样品中核苷酸的测定。

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