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首页> 外文期刊>Chromatography >Studying Plant–Insect Interactions with Solid Phase Microextraction: Screening for Airborne Volatile Emissions Response of Soybeans to the Soybean Aphid, Aphis glycines Matsumura (Hemiptera: Aphididae)
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Studying Plant–Insect Interactions with Solid Phase Microextraction: Screening for Airborne Volatile Emissions Response of Soybeans to the Soybean Aphid, Aphis glycines Matsumura (Hemiptera: Aphididae)

机译：用固相微萃取研究植物与昆虫的相互作用：筛选大豆对大豆蚜虫，蚜虫甘氨酸的空气传播的挥发性排放物的反应松村（Hemiptera：Aphididae）

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Insects trigger plants to release volatile compounds that mediate the interaction with both pest and beneficial insects. Soybean aphids (Aphis glycines) induces soybean (Glycine max) leaves to produce volatiles that attract predators of the aphid. In this research, we describe the use of solid-phase microextraction (SPME) for extraction of volatiles from A. glycines-infested plant. Objectives were to (1) determine if SPME can be used to collect soybean plant volatiles and to (2) use headspace SPME-GC-MS approach to screen compounds associated with A. glycines-infested soybeans, grown in the laboratory and in the field, to identify previously known and potentially novel chemical markers of infestation. A total of 62 plant volatiles were identified, representing 10 chemical classes. 39 compounds had not been found in previous studies of soybean volatile emissions. 3-hexen-1-ol, dimethyl nonatriene, indole, caryophyllene, benzaldehyde, linalool, methyl salicylate (MeSA), benzene ethanol, and farnesene were considered herbivore-induced plant volatiles (HIPVs). For reproductive field-grown soybeans, three compounds were emitted in greater abundance from leaves infested with A. glycines, cis-3-hexen-1-ol acetate, MeSA and farnesene. In summary, SPME can detect the emission of HIPVs from plants infested with insect herbivores.
机译：昆虫触发植物释放挥发性化合物，介导与害虫和有益昆虫的相互作用。大豆蚜虫（Aphis glycines）诱导大豆（Glycine max）叶片产生挥发物，吸引了蚜虫的天敌。在这项研究中，我们描述了使用固相微萃取（SPME）从甘草曲霉感染的植物中提取挥发物的方法。目的是（1）确定SPME是否可用于收集大豆植物挥发物，以及（2）使用顶空SPME-GC-MS方法筛选与在实验室和田间生长的被甘氨酸浸染的大豆相关的化合物，以确定以前已知的和潜在的新颖的侵扰性化学标记。总共鉴定出62种植物挥发物，代表10种化学类别。在以前的大豆挥发物排放研究中未发现39种化合物。 3-己烯-1-醇，二甲基壬二烯，吲哚，石竹烯，苯甲醛，芳樟醇，水杨酸甲酯（MeSA），苯乙醇和法呢烯被认为是草食动物诱导的植物挥发物（HIPVs）。对于繁殖田间种植的大豆，富含A.甘氨酸，顺式3-己烯-1-醇乙酸酯，MeSA和法呢烯的叶片中释放出三种化合物。综上所述，SPME可以检测出被昆虫食草动物侵染的植物中HIPV的排放。

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