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首页> 外文期刊>CHROMATOGRAPHY >Effect of Acidic Additives on Peak Capacity and Detectivity in Peptide Analysis Using Nano-Flow LC/MS with Low-Density ODS Modified Monolithic Silica Capillary Columns
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Effect of Acidic Additives on Peak Capacity and Detectivity in Peptide Analysis Using Nano-Flow LC/MS with Low-Density ODS Modified Monolithic Silica Capillary Columns

机译：酸性添加剂对低密度ODS修饰的整体硅胶毛细管色谱柱纳流LC / MS进行肽分析时峰容量和检测能力的影响

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This report describes the relation between peak capacity and mass spectrometric detectivity on peptides analysis with low-density octadecylsilylated monolithic silica capillary columns for several acidic additives in mobile phase using a nano-flow LC/MS. As the acidic additives, trifluoroacetic acid (TFA), 3,3,3-trifluoropropionic acid (TriFPA), formic acid (FA), acetic acid (AA), and cyanoacetic acid (CAA) were evaluated. Peak capacity and mass spectrometric detectivity were evaluated by using the peptides mixture in the gradient elution of water/acetonitrile with 0.1% (v/v) additives at 1-50% acetonitrile composition for 35 minutes on low-density octadecylsilylated monolithic silica capillary columns. Peak capacity is usually estimated by using the ratio of gradient time to average peak width of peptides. However, we estimated the peak capacity by using the ratio of t _(0) to the time of final peak to average peak width of peptides because the retention time of peptides was varied by difference of additives. As a result, peak capacity was increased by utilizing higher acidity additives, while the mass spectrometric detectivity was decreased. This result suggested that both high peak capacity and high detectivity for peptides analysis is irreconcilable. To overcome this relation, the impact of CAA was investigated because of its thermolysis behavior. In the condition of mobile phase with CAA at high temperature condition of mass spectrometry capillary inlet, higher peak capacity than those with TriFPA and even detectivity for those with FA were observed.
机译：本报告介绍了使用纳流液相色谱/质谱联用低密度十八烷基甲硅烷基化整体硅胶毛细管色谱柱对流动相中的几种酸性添加剂进行肽分析时，峰容量与质谱检测率之间的关系。作为酸性添加剂，对三氟乙酸（TFA），3,3,3-三氟丙酸（TriFPA），甲酸（FA），乙酸（AA）和氰基乙酸（CAA）进行了评价。在低密度十八烷基甲硅烷基化整体硅胶毛细管柱上使用肽混合物在1-50％乙腈组成的水/乙腈与0.1％（v / v）添加剂的梯度洗脱中进行35分钟，以评估峰容量和质谱检测度。通常使用梯度时间与肽的平均峰宽之比估算峰容量。但是，由于肽的保留时间会因添加剂的不同而变化，因此我们通过使用 t _（0）与肽的最终峰时间与平均峰宽之比来估算峰容量。结果，通过使用较高酸度的添加剂增加了峰容量，而降低了质谱检测率。该结果表明，用于肽分析的高峰容量和高检测能力都是不可调和的。为了克服这种关系，研究了CAA的热解行为，从而对其产生了影响。在质谱毛细管毛细管入口的高温条件下，在使用CAA流动相的条件下，观察到的峰容量高于使用TriFPA的峰容量，甚至可以检测到使用FA的峰。

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