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Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

机译：使用插装式整体硅胶柱进行氨基酸分析

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In this study, the application of a new type of monolithic silica column to the analysis of amino acids in human plasma samples has been investigated. The monolithic silica column (3.0 × 150 mm, MonoTower C18) provided good separation of the amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). Although the separation ability of this column was not much improved in comparison with the previous monolithic silica columns, it was similar with that of a 3 μm particle packed column. The column with length 150 mm had the same separation property as a tandem connection of columns of lengths 50 mm and 100 mm. After 1000 injections of human plasma samples, the peak widths for the NBD-amino acids were increased by only 8%. Thus, the newly developed monolithic silica column can be potentially applied for faster analysis and/or more efficient separation of biological compounds.
机译：在这项研究中，已经研究了一种新型整体硅胶柱在分析人体血浆样品中氨基酸方面的应用。整体式硅胶柱（3.0×150 mm，MonoTower C18）可以很好地分离用4-氟-7-硝基-2,1,3-苯并恶二唑（NBD-F）衍生的氨基酸。尽管与以前的整体式硅胶色谱柱相比，该色谱柱的分离能力没有太大提高，但与3μm颗粒填充色谱柱的分离能力相似。长度为150 mm的色谱柱与串联连接的长度为50 mm和100 mm的色谱柱具有相同的分离特性。人体血浆样品注射1000次后，NBD氨基酸的峰宽仅增加了8％。因此，新开发的整体式硅胶柱可潜在地用于更快地分析和/或更有效地分离生物化合物。

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