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首页> 外文期刊>CHROMATOGRAPHY >Expanding the Versatility of a Quantitative Determination Range Adjustment Technique Using In-Source CID in LC/MS/MS
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Expanding the Versatility of a Quantitative Determination Range Adjustment Technique Using In-Source CID in LC/MS/MS

机译：在LC / MS / MS中使用源内CID扩展定量测定范围调整技术的多功能性

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The aim of this study was to investigate the applicability of a new technique using in-source collision-induced dissociation (CID) for improving quantitative linear range of various compounds in liquid chromatography-tandem mass spectrometry (LC/MS/MS). To determine whether the linear range shift due to in-source CID occurs under various MS conditions, we investigated the quantitative linear ranges of reserpine, indomethacin, and furosemide in both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in positive- and negative-ion detection modes. We observed 3–30-fold linear range shifts upon changing the declustering potential to adjust in-source CID in positive- and negative-ion detection modes in both ESI and APCI. These results indicated that this new technique could be applied for arbitrary adjustment of linear range regardless of ionization process and polarities in LC/MS/MS. Therefore, this technique could be applied to simultaneous quantification, in biological fluids, of compounds with quite different sensitivities under various MS conditions.
机译：这项研究的目的是研究一种使用源内碰撞诱导解离（CID）的新技术在液相色谱-串联质谱（LC / MS / MS）中改善各种化合物的定量线性范围的适用性。为了确定在不同的质谱条件下是否发生源内CID引起的线性范围变化，我们研究了正电子显微镜下电喷雾电离（ESI）和大气压化学电离（APCI）中利血平，消炎痛和呋塞米的定量线性范围。和负离子检测模式。我们观察到在ESI和APCI中，改变消簇电位以调整源内CID时在正离子和负离子检测模式下的线性变化范围为3-30倍。这些结果表明，该新技术可应用于线性范围的任意调节，而与LC / MS / MS中的电离过程和极性无关。因此，该技术可用于同时在生物液体中定量测定在各种质谱条件下灵敏度差异很大的化合物。

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