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首页> 外文期刊>CHROMATOGRAPHY >Sensitivity Enhancement by Sweeping via Solid Phase Extraction Using Titania Nanoparticles in Capillary Electrophoretic Analysis of Phosphopeptides
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Sensitivity Enhancement by Sweeping via Solid Phase Extraction Using Titania Nanoparticles in Capillary Electrophoretic Analysis of Phosphopeptides

机译：通过二氧化钛纳米粒子通过固相萃取扫掠i进行磷酸肽的毛细管电泳分析，从而提高灵敏度。

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摘要

This paper reports an on-line sample preconcentration by a sweeping technique based on the surface complexation between TiO_(2) (titania) nanoparticles and phosphate groups in the capillary electrophoresis (CE) analysis of phosphopeptides. It is well-known that titania particles can trap phosphopeptides under an acidic condition, while under an alkaline condition they are eluted from the titania surfaces. In the sweeping via solid phase extraction using the titania nanoparticles, a phosphopeptide solution is injected as a long plug into a capillary filled with an acidic background solution. Due to the complexation onto the titania surfaces, the analytes in a long sample zone are swept by the titania particles to a narrow zone. This preconcentration technique was applied to the analysis of monophosphopeptide from β-casein. When an 1% titania dispersion and 100 ppm phosphopeptide solution was successively introduced into the capillary at the injection time of 30 s and 60 s, respectively, a sharp peak of the phosphopeptide was obtained by UV detection without any optical interferences of the titania. Comparing with a conventional CE analysis, a 22-fold sensitivity increase was achieved by the preconcentration effect.
机译：本文报告了基于吹扫技术的在线样品预富集，该技术基于磷酸肽的毛细管电泳（CE）分析中TiO_（2）（二氧化钛）纳米粒子与磷酸基团之间的表面络合。众所周知，二氧化钛颗粒可以在酸性条件下捕获磷酸肽，而在碱性条件下它们可以从二氧化钛表面上洗脱下来。在使用二氧化钛纳米颗粒通过固相萃取的扫掠中，将磷酸肽溶液作为长塞注入到填充有酸性背景溶液的毛细管中。由于络合到二氧化钛表面上，长样品区域中的分析物被二氧化钛颗粒吹扫到狭窄区域。该预浓缩技术用于分析β-酪蛋白中的单磷酸肽。当分别在30 s和60 s的注射时间分别将1％的二氧化钛分散液和100 ppm磷酸肽溶液引入毛细管时，通过紫外检测获得了磷酸肽的尖峰，而二氧化钛没有任何光学干扰。与传统的CE分析相比，预浓缩效应使灵敏度提高了22倍。

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