• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cilia >Non-invasive sources of cells with primary cilia from pediatric and adult patients
【24h】

Non-invasive sources of cells with primary cilia from pediatric and adult patients

机译：小儿和成年患者原发性纤毛细胞的非侵入性来源

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Ciliopathies give rise to a multitude of organ-specific pathologies; obtaining relevant primary patient material is useful for both diagnostics and research. However, acquisition of primary ciliated cells from patients, particularly pediatric patients, presents multiple difficulties. Biopsies and blood samples are invasive, and patients (and their parents) may be reluctant to travel to medical centers, especially for research purposes. We sought to develop non-invasive methods of obtaining viable and ciliated primary cells from ciliopathy patients which could be obtained in the home environment. Findings We introduce two methods for the non-invasive acquisition of primary ciliated cells. In one approach, we collected spontaneously shed deciduous (milk) teeth from children. Fibroblast-like cells were observed after approximately 2 weeks of culture of fragmented teeth. Secondly, urine samples were collected from children or adults. Cellular content was isolated and after approximately 1 week, renal epithelial cells were observed. Both urine and tooth-derived cells ciliate and express ciliary proteins visible with immunofluorescence. Urine-derived renal epithelial cells (URECs) are amenable to 3D culturing, siRNA knockdown, and ex vivo drug testing. Conclusions As evidence continues to accumulate showing that the primary cilium has a central role in development and disease, the need for readily available and ciliated patient cells will increase. Here, we introduce two methods for the non-invasive acquisition of cells with primary cilia. We believe that these cells can be used for further ex vivo study of ciliopathies and in the future, for personalized medicine.
机译：背景脊髓型颈椎病引起多种器官特有的病理学。获得相关的主要患者材料对于诊断和研究都是有用的。然而，从患者，特别是儿科患者获得原代纤毛细胞存在多种困难。活检和血液样本具有侵入性，患者（及其父母）可能不愿前往医疗中心，特别是出于研究目的。我们试图开发一种非侵入性方法，可以从家庭环境中获得从睫状肌病患者那里获得存活的和纤毛的原代细胞。研究结果我们介绍了两种非侵入性获取原代纤毛细胞的方法。在一种方法中，我们收集了儿童自发脱落的乳齿。培养大约2周的牙齿碎片后，观察到成纤维细胞样细胞。其次，从儿童或成人收集尿液样本。分离细胞含量，大约1周后，观察到肾上皮细胞。尿液和牙齿来源的细胞均可发生纤毛并表达具有免疫荧光可见的纤毛蛋白。尿液衍生的肾上皮细胞（UREC）适用于3D培养，siRNA抑制和离体药物测试。结论随着越来越多的证据表明初级纤毛在发育和疾病中起着重要作用，对容易获得和纤毛的患者细胞的需求将会增加。在这里，我们介绍了无创获取原发性纤毛细胞的两种方法。我们相信，这些细胞可用于进一步对纤毛虫进行离体研究，并在将来用于个性化医学。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号