...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Circulation journal >Global Investigation of Immune Repertoire Suggests Kawasaki Disease Has Infectious Cause
【24h】

Global Investigation of Immune Repertoire Suggests Kawasaki Disease Has Infectious Cause

机译：免疫库的全球调查表明，川崎病具有传染性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Kawasaki disease (KD) severely threatens young children’s health worldwide. The pathogenic mechanism of KD has not yet been solved, so there is still debate over whether KD is an infectious disease or an autoimmune disease. Methods?and?Results: To solve this problem, an immune repertoire analysis of KD was conducted. We collected blood cell RNA samples and prepared them into amplicons with iRepertoire kits. The amplicons were sequenced and analyzed with the iRepertoire pipeline. We first identified KD-specific VJ and VDJ forms that had the potential to serve as biomarkers of KD. In addition, the KD-specific VDJ forms were contributed mostly by immunoglobulin G. The D50 value analysis showed that B-cell diversity in KD is decreased, suggesting unique immunoglobulins are produced in KD. Moreover, V, D and J segment usage in IgA, IgG and IgM was consistent with previous KD studies. Further comparison showed no difference in CDR3 peptide length between KD and fever controls (subjects with fever but not diagnosed as KD), indicting KD had B-cell selection phenomenon that has a non-autoimmune pattern. The comparison of amino acid usage of the CDR3 region demonstrated a preference for hydrophilic amino acids in KD. Conclusions: The results of D50 value, VDJ usage and CDR3 peptide length analyses suggested the characteristics of infectious disease for KD.
机译：背景：川崎病（KD）严重威胁着全球幼儿的健康。 KD的致病机制尚未解决，因此关于KD是传染病还是自身免疫病仍存在争议。方法和结果：为了解决这个问题，对KD进行了免疫库分析。我们收集了血细胞RNA样品，并使用iRepertoire试剂盒将其制备为扩增子。对扩增子进行测序，并通过iRepertoire管道进行分析。我们首先确定了KD特异性的VJ和VDJ形式，这些形式有可能用作KD的生物标记。此外，KD特异性VDJ形式主要由免疫球蛋白G贡献。D50值分析表明KD中的B细胞多样性降低，这表明KD中产生了独特的免疫球蛋白。此外，IgA，IgG和IgM中V，D和J段的使用与以前的KD研究一致。进一步的比较显示，KD和发烧对照（发烧但未诊断为KD的受试者）之间的CDR3肽长度没有差异，这表明KD具有非自身免疫模式的B细胞选择现象。 CDR3区氨基酸使用情况的比较表明，KD中偏爱亲水性氨基酸。结论：D50值，VDJ使用情况和CDR3肽长度分析的结果提示了KD的传染病特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号