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首页> 外文期刊>Clinical and vaccine immunology: CVI >Optimization and Application of a Multiplex Bead-Based Assay To Quantify Serotype-Specific IgG against Streptococcus pneumoniae Polysaccharides: Response to the Booster Vaccine after Immunization with the Pneumococcal 7-Valent Conjugate Vaccine
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Optimization and Application of a Multiplex Bead-Based Assay To Quantify Serotype-Specific IgG against Streptococcus pneumoniae Polysaccharides: Response to the Booster Vaccine after Immunization with the Pneumococcal 7-Valent Conjugate Vaccine

机译：优化和应用基于多重珠的测定方法来量化针对肺炎链球菌多糖的血清型特异性IgG：肺炎球菌7价共轭疫苗免疫后对加强疫苗的反应

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摘要

We describe the optimization and application of a multiplex bead-based assay (Luminex) to quantify antibodies against polysaccharides of 13 pneumococcal serotypes. In the optimized multiplex immunoassay (MIA), intravenous immune globulin was introduced as an in-house reference serum, and nonspecific reacting antibodies were adsorbed with the commercial product pneumococcal C polysaccharides Multi. The antibody concentrations were assessed in 188 serum samples obtained pre- and post-booster vaccination at 11 months after administration of a primary series of the pneumococcal seven-valent conjugate vaccine (PCV-7) at 2, 3, and 4 months of age. The results of the MIA were compared with those of the ELISA for the serotypes included in the seven-valent conjugated polysaccharide vaccine and for a non-vaccine serotype, serotype 6A. The geometric mean concentrations of the antibodies determined by MIA were slightly higher than those determined by ELISA. The correlations between the assays were good, with R2 values ranging from 0.84 to 0.91 for all serotypes except serotype 19F, for which R2 was 0.70. The concentrations of antibody against serotype 6A increased after the administration of PCV-7 due to cross-reactivity with serotype 6B. The differences between the results obtained by ELISA and MIA suggest that the internationally established protective threshold of 0.35 μg/ml should be reevaluated for use in the MIA and may need to be amended separately for each serotype.
机译：我们描述了基于多重磁珠的测定法（Luminex）的优化和应用，以定量针对13种肺炎球菌血清型多糖的抗体。在优化的多重免疫测定（MIA）中，引入了静脉内免疫球蛋白作为内部参考血清，并且非特异性反应抗体被商品化肺炎球菌C多糖Multi吸附。在接种2、3和4个月大的肺炎球菌7价初级疫苗系列后11个月，在加强免疫接种前后的188个血清样本中评估了抗体浓度。对于包含在七价结合多糖疫苗中的血清型和用于非疫苗血清型6A的血清型，将MIA的结果与ELISA的结果进行了比较。通过MIA测定的抗体的几何平均浓度略高于通过ELISA测定的浓度。这些测定之间的相关性很好，除19F血清型（ R R 2 值在0.84至0.91之间> 2 为0.70。由于与血清型6B的交叉反应，在给予PCV-7后，针对血清型6A的抗体的浓度增加。 ELISA和MIA所得结果之间的差异表明，应重新评估国际认可的0.35μg/ ml保护阈值，以用于MIA，并且可能需要针对每种血清型分别进行修改。

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