• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and vaccine immunology: CVI >Cell-Mediated and Humoral Immune Responses after Immunization of Calves with a Recombinant Multiantigenic Mycobacterium avium subsp. paratuberculosis Subunit Vaccine at Different Ages
【24h】

Cell-Mediated and Humoral Immune Responses after Immunization of Calves with a Recombinant Multiantigenic Mycobacterium avium subsp. paratuberculosis Subunit Vaccine at Different Ages

机译：用重组多抗原鸟分枝杆菌亚种免疫小牛后的细胞介导和体液免疫反应。不同年龄的肺结核旁亚单位疫苗

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Neonates and juvenile ruminants are very susceptible to paratuberculosis infection. This is likely due to a high degree of exposure from their dams and an immature immune system. To test the influence of age on vaccine-induced responses, a cocktail of recombinant Mycobacterium avium subsp. paratuberculosis proteins (MAP0217, MAP1508, MAP3701c, MAP3783, and MAP1609c/Ag85B) was formulated in a cationic liposome adjuvant (CAF01) and used to vaccinate animals of different ages. Male jersey calves were divided into three groups that were vaccinated at 2, 8, or 16 weeks of age and boosted twice at weeks 4 and 12 relative to the first vaccination. Vaccine-induced immune responses, the gamma interferon (IFN-γ) cytokine secretion and antibody responses, were followed for 20 weeks. In general, the specific responses were significantly elevated in all three vaccination groups after the first booster vaccination with no or only a minor effect from the second booster. However, significant differences were observed in the immunogenicity levels of the different proteins, and it appears that the older age group produced a more consistent IFN-γ response. In contrast, the humoral immune response is seemingly independent of vaccination age as we found no difference in the IgG1 responses when we compared the three vaccination groups. Combined, our results suggest that an appropriate age of vaccination should be considered in vaccination protocols and that there is a possible interference of vaccine-induced immune responses with weaning (week 8).
机译：新生儿和少年反刍动物很容易感染副结核病。这可能是由于它们的母坝高度暴露以及免疫系统未成熟造成的。为了测试年龄对疫苗诱导反应的影响，重组鸟分枝杆菌亚种的混合物。将副结核病蛋白（MAP0217，MAP1508，MAP3701c，MAP3783和MAP1609c / Ag85B）配制在阳离子脂质体佐剂（CAF01）中，并用于给不同年龄的动物接种疫苗。将雄性泽西犊牛分为三组，分别在2、8、16周龄时接种疫苗，并相对于第一次疫苗接种在4周和12周时加强免疫两次。疫苗诱导的免疫反应，γ干扰素（IFN-γ）细胞因子分泌和抗体反应持续20周。通常，在第一次加强免疫后，所有三个疫苗接种组的特异性反应均显着升高，而第二次加强免疫则没有或只有很小的作用。然而，在不同蛋白质的免疫原性水平上观察到显着差异，并且似乎老年组产生了更一致的IFN-γ应答。相反，体液免疫应答似乎与疫苗接种年龄无关，因为当我们比较三个疫苗接种组时，我们发现IgG1应答没有差异。综合而言，我们的结果表明，在接种方案中应考虑适当的接种年龄，并且断奶可能会干扰疫苗诱导的免疫反应（第8周）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号