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首页> 外文期刊>Clinical and vaccine immunology: CVI >Expression and Secretion of Cathelicidin LL-37 in Human Epithelial Cells after Infection by Mycobacterium bovis Bacillus Calmette-Guérin
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Expression and Secretion of Cathelicidin LL-37 in Human Epithelial Cells after Infection by Mycobacterium bovis Bacillus Calmette-Guérin

机译：Cathelicidin LL-37在牛分枝杆菌卡介苗的感染后在人上皮细胞中的表达和分泌

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The antimicrobial cathelicidin LL-37 is considered to play an important role in the innate immune response to tuberculosis infection. However, little is known about the induction and secretion of this antimicrobial peptide in A549 epithelial cells after infection with Mycobacterium bovis bacillus Calmette-Guérin (BCG), the world's most widely used tuberculosis vaccine. In this study, we investigated the effect of M. bovis BCG on LL-37 mRNA levels in A549 cells by real-time PCR and on protein levels by Western blotting. Treatment of cells with M. bovis BCG upregulates LL-37 mRNA expression in a dose- and time-dependent manner. The quantitative analysis of LL-37 gene expression correlated with our Western blotting results. Moreover, our results demonstrated that treatment of cells with the transcriptional inhibitor actinomycin D effectively inhibited in a concentration-dependent manner the ability of M. bovis BCG to induce LL-37 mRNA expression. Finally, inhibition of the MEK1/2 and p38 mitogen-activated protein kinase (MAPK) signaling pathways reduced M. bovis BCG-mediated LL-37 mRNA expression, a reduction that correlated with the observed high level of downregulation of LL-37 protein induction. Thus, these results indicate that the MEK1/2 and p38 MAPK signaling pathways play a critical role in the regulation of inducible LL-37 gene expression in A549 cells infected with M. bovis BCG.
机译：抗菌素Cathelicidin LL-37被认为在对结核感染的先天免疫应答中起着重要作用。然而，关于这种抗微生物肽在被世界上使用最广泛的结核病疫苗<分枝杆菌>卡门特-盖林（BCG）感染后在A549上皮细胞中的诱导和分泌知之甚少。在这项研究中，我们调查了 M的影响。实时PCR检测牛卡介苗卡介苗对A549细胞LL-37 mRNA表达的影响，Western blotting检测牛血清对蛋白水平的影响。用 M处理细胞。牛卡介苗BCG以剂量和时间依赖性方式上调LL-37 mRNA的表达。 LL-37基因表达的定量分析与我们的蛋白质印迹结果相关。此外，我们的结果表明，用转录抑制剂放线菌素D处理细胞可有效地以浓度依赖性方式抑制 M的能力。牛BCG诱导LL-37 mRNA表达。最后，MEK1 / 2和p38丝裂原活化蛋白激酶（MAPK）信号通路的抑制降低了 M。 bovis BCG介导的LL-37 mRNA表达的降低，与观察到的LL-37蛋白诱导的高水平下调相关。因此，这些结果表明，MEK1 / 2和p38MAPK信号传导途径在感染 M的A549细胞中可诱导的LL-37基因表达的调节中起关键作用。 bovis BCG。

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