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首页> 外文期刊>Clinical and vaccine immunology: CVI >Diagnosis of Bovine Paratuberculosis by a Novel Enzyme-Linked Immunosorbent Assay Based on Early Secreted Antigens of Mycobacterium avium subsp. paratuberculosis
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Diagnosis of Bovine Paratuberculosis by a Novel Enzyme-Linked Immunosorbent Assay Based on Early Secreted Antigens of Mycobacterium avium subsp. paratuberculosis

机译：基于鸟分枝杆菌亚种早期分泌抗原的新型酶联免疫吸附法诊断牛副结核病。副结核病

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We previously reported that protein antigens of serodiagnostic potential were more abundant in culture filtrates than cellular extracts from liquid cultures of Mycobacterium avium subsp. paratuberculosis (D. Cho and M. T. Collins, Clin. Vaccine Immunol. 13:1155-1161, 2006). Based on this observation, a novel enzyme-linked immunosorbent assay (ELISA) using antigens secreted by young (early- to mid-log-phase) cultures of M. avium subsp. paratuberculosis JTC303 (a low-passage isolate originating from the ileum of a Holstein bull) in mycobactin-supplemented Watson-Reid medium (pH 6.0) was developed and evaluated using a previously described panel of bovine sera (M. T. Collins et al., Clin. Diagn. Lab. Immunol. 12:685-692, 2005) that included 444 paratuberculosis cases and 412 controls. The new assay, called JTC-ELISA, had a significantly higher diagnostic sensitivity and an equivalent specificity compared to those of five commercial paratuberculosis ELISA kits. By receiver-operating characteristic analysis, the JTC-ELISA had the highest area under the curve of the six assays evaluated. The JTC-ELISA was particularly sensitive at detecting low-level fecal shedders of Mavium subsp. paratuberculosis (40%; the sensitivity of the commercial kits was 20%). The JTC-ELISA works effectively on both serum and milk samples for the detection of cattle with subclinical M. avium subsp. paratuberculosis infections, providing a cost-effective diagnostic tool to support paratuberculosis control programs in cattle herds.
机译：我们以前曾报道过，血清学诊断潜力的蛋白质抗原在培养滤液中比从鸟分枝杆菌亚种的液体培养物中提取的细胞提取物丰富。 副结核病（D. Cho和M. T. Collins，临床疫苗免疫，13：1155-1161，2006）。基于此观察结果，我们使用了一种新型酶联免疫吸附测定（ELISA），该酶利用了 M的年轻（早期至对数中期）培养物分泌的抗原。 avium 子空间开发了在补充霉菌素的沃森-里德（Watson-Reid）培养基（pH 6.0）中的副结核 JTC303（源自荷斯坦公牛回肠的低通道分离株），并使用先前描述的牛血清（MT）进行了评估Collins等人（Clin。Diagn。Lab。Immunol。12：685-692，2005），其中包括444例肺结核病例和412例对照。与五种商业化的副结核ELISA试剂盒相比，这种称为JTC-ELISA的新方法具有更高的诊断敏感性和同等的特异性。通过接受者操作特征分析，JTC-ELISA在所评估的六种测定法的曲线下面积最大。 JTC-ELISA对检测 Mavium 亚种的低水平粪便粪便特别敏感。 肺结核（占40％;市售试剂盒的敏感性为20％）。 JTC-ELISA对血清和牛奶样品均有效，可用于检测亚临床 M的牛。 avium 子空间肺结核感染，提供了一种经济有效的诊断工具来支持牛群中的肺结核旁控制计划。

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