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首页> 外文期刊>Clinical and vaccine immunology: CVI >Measurement of the Humoral Immune Response following an Incident Human Papillomavirus Type 16 or 18 Infection in Young Women by a Pseudovirion-Based Neutralizing Antibody Assay
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Measurement of the Humoral Immune Response following an Incident Human Papillomavirus Type 16 or 18 Infection in Young Women by a Pseudovirion-Based Neutralizing Antibody Assay

机译：基于假病毒颗粒的中和抗体检测年轻妇女中发生人乳头瘤病毒16型或18型感染后的体液免疫反应的测量

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摘要

We have evaluated a neutralizing antibody assay which uses human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 pseudovirions carrying a secretory alkaline phosphatase reporter gene and which can potentially measure functionally relevant HPV type-specific neutralizing antibodies. The reproducibility of the assay was excellent; for HPV-16, the intra- and interassay kappa values were 0.95 and 0.90, respectively; and for HPV-18, the corresponding values were 0.90 and 0.90. This assay was used to describe the kinetics of the neutralizing antibody response in a cohort of 42 young women who were recruited soon after first intercourse and who first tested positive for HPV-16 DNA or HPV-18 DNA, or both, during follow-up. Most women seroconverted following the first detection of type-specific HPV DNA and remained seropositive until the end of follow-up. Our findings are broadly consistent with those of two other cohort studies which have measured the serological response following an incident infection by using the technically simpler virus-like-particle-based enzyme-linked immunosorbent assay.
机译：我们评估了中和抗体测定法，该测定法使用携带分泌碱性磷酸酶报道基因的人乳头瘤病毒（HPV）16型（HPV-16）和HPV-18假病毒粒子，并且可以潜在地测量功能相关的HPV类型特异性中和抗体。该方法的重现性极佳;对于HPV-16，测定内和测定间卡巴值分别为0.95和0.90;对于HPV-18，相应的值为0.90和0.90。该试验用于描述一组42名年轻女性的中和抗体应答的动力学，这些女性在第一次性交后不久就被招募，并且在随访期间首次检测出HPV-16 DNA或HPV-18 DNA或两者均为阳性。在首次检测到特定类型的HPV DNA之后，大多数女性会发生血清转变，并且在随访结束之前一直保持血清阳性。我们的发现与其他两项队列研究的结果基本一致，该两项研究通过使用技术上更简单的基于病毒样颗粒的酶联免疫吸附测定法测量了事件感染后的血清学反应。

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